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PMID:17548512

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Citation

Besse, F, Mertel, S, Kittel, RJ, Wichmann, C, Rasse, TM, Sigrist, SJ and Ephrussi, A (2007) The Ig cell adhesion molecule Basigin controls compartmentalization and vesicle release at Drosophila melanogaster synapses. J. Cell Biol. 177:843-55

Abstract

Synapses can undergo rapid changes in size as well as in their vesicle release function during both plasticity processes and development. This fundamental property of neuronal cells requires the coordinated rearrangement of synaptic membranes and their associated cytoskeleton, yet remarkably little is known of how this coupling is achieved. In a GFP exon-trap screen, we identified Drosophila melanogaster Basigin (Bsg) as an immunoglobulin domain-containing transmembrane protein accumulating at periactive zones of neuromuscular junctions. Bsg is required pre- and postsynaptically to restrict synaptic bouton size, its juxtamembrane cytoplasmic residues being important for that function. Bsg controls different aspects of synaptic structure, including distribution of synaptic vesicles and organization of the presynaptic cortical actin cytoskeleton. Strikingly, bsg function is also required specifically within the presynaptic terminal to inhibit nonsynchronized evoked vesicle release. We thus propose that Bsg is part of a transsynaptic complex regulating synaptic compartmentalization and strength, and coordinating plasma membrane and cortical organization.

Links

PubMed PMC2064284 Online version:10.1083/jcb.200701111

Keywords

Actin Cytoskeleton/metabolism; Actin Cytoskeleton/ultrastructure; Amino Acid Sequence; Animals; Cell Adhesion; Cell Compartmentation; Down-Regulation; Drosophila Proteins/chemistry; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Drosophila melanogaster/cytology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Membrane Glycoproteins/chemistry; Membrane Glycoproteins/genetics; Membrane Glycoproteins/physiology; Molecular Sequence Data; Mutation; Neuromuscular Junction/metabolism; Protein Structure, Tertiary; Sequence Alignment; Synapses/metabolism; Synapses/physiology; Synaptic Vesicles/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:M9PCW5

located_in

GO:0048788: cytoskeleton of presynaptic active zone

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:M9PCW5

located_in

GO:0048786: presynaptic active zone

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:M9PCW5

involved_in

GO:0098609: cell-cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q7KTJ7

located_in

GO:0048788: cytoskeleton of presynaptic active zone

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q7KTJ7

located_in

GO:0048786: presynaptic active zone

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q7KTJ7

involved_in

GO:0098609: cell-cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8IPG9

located_in

GO:0048788: cytoskeleton of presynaptic active zone

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8IPG9

located_in

GO:0048786: presynaptic active zone

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8IPG9

involved_in

GO:0098609: cell-cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q961I4

located_in

GO:0048788: cytoskeleton of presynaptic active zone

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q961I4

located_in

GO:0048786: presynaptic active zone

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q961I4

involved_in

GO:0098609: cell-cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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