PMID:17541406

Citation	Pilo Boyl, P, Di Nardo, A, Mulle, C, Sassoè-Pognetto, M, Panzanelli, P, Mele, A, Kneussel, M, Costantini, V, Perlas, E, Massimi, M, Vara, H, Giustetto, M and Witke, W (2007) Profilin2 contributes to synaptic vesicle exocytosis, neuronal excitability, and novelty-seeking behavior. EMBO J. 26:2991-3002
Abstract	Profilins are actin binding proteins essential for regulating cytoskeletal dynamics, however, their function in the mammalian nervous system is unknown. Here, we provide evidence that in mouse brain profilin1 and profilin2 have distinct roles in regulating synaptic actin polymerization with profilin2 preferring a WAVE-complex-mediated pathway. Mice lacking profilin2 show a block in synaptic actin polymerization in response to depolarization, which is accompanied by increased synaptic excitability of glutamatergic neurons due to higher vesicle exocytosis. These alterations in neurotransmitter release correlate with a hyperactivation of the striatum and enhanced novelty-seeking behavior in profilin2 mutant mice. Our results highlight a novel, profilin2-dependent pathway, regulating synaptic physiology, neuronal excitability, and complex behavior.
Links	PubMed PMC1894775 Online version:10.1038/sj.emboj.7601737
Keywords	Animals; Behavior, Animal/physiology; Exocytosis/physiology; Learning; Long-Term Potentiation; Memory; Mice; Neurons/physiology; Profilins/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:PROF2	acts_upstream_of_or_within	GO:0030838: positive regulation of actin filament polymerization	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:3715145	P		Seeded From UniProt	complete
MOUSE:PROF2	acts_upstream_of_or_within	GO:2000300: regulation of synaptic vesicle exocytosis	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:3715145	P		Seeded From UniProt	complete
MOUSE:PROF2	part_of	GO:0098794: postsynapse	ECO:0006003: electron microscopy evidence used in manual assertion		C	part_of:(GO:0098685)	Seeded From UniProt	complete
MOUSE:PROF2	involved_in	GO:2000300: regulation of synaptic vesicle exocytosis	ECO:0001225: knockout evidence used in manual assertion		P	occurs_in:(GO:0098978) occurs_in:(UBERON:0002435)	Seeded From UniProt	complete
MOUSE:PROF2	involved_in	GO:2000300: regulation of synaptic vesicle exocytosis	ECO:0006013: patch-clamp recording evidence used in manual assertion		P	occurs_in:(GO:0098978) occurs_in:(UBERON:0002435)	Seeded From UniProt	complete
MOUSE:PROF2	part_of	GO:0098685: Schaffer collateral - CA1 synapse	ECO:0006003: electron microscopy evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:PROF2	part_of	GO:0098978: glutamatergic synapse	ECO:0001225: knockout evidence used in manual assertion		C	part_of:(UBERON:0002435)	Seeded From UniProt	complete
MOUSE:PROF2	part_of	GO:0098978: glutamatergic synapse	ECO:0006013: patch-clamp recording evidence used in manual assertion		C	part_of:(UBERON:0002435)	Seeded From UniProt	complete
MOUSE:PROF2	part_of	GO:0098793: presynapse	ECO:0006003: electron microscopy evidence used in manual assertion		C	part_of:(GO:0098685)	Seeded From UniProt	complete
MOUSE:DYN1	part_of	GO:0043195: terminal bouton	ECO:0000314: direct assay evidence used in manual assertion		C	part_of:(EMAPA:16894)	Seeded From UniProt	complete

References

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