GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17540517

From GONUTS
Jump to: navigation, search
Citation

Borrmann, E, Berndt, A, Hänel, I and Köhler, H (2007) Campylobacter-induced interleukin-8 responses in human intestinal epithelial cells and primary intestinal chick cells. Vet. Microbiol. 124:115-24

Abstract

Campylobacter (C.) jejuni and C. coli can cause gastrointestinal disorders in humans characterized by acute inflammation. Inflammatory signals are initiated during interaction between these pathogens and human intestinal cells, but nothing is known about the stimulation of avian intestinal cells by Campylobacter. Interleukin-8 (IL-8) as a proinflammatory chemokine plays an important role in mobilizing cellular defence mechanism. IL-8 mRNA expression in both human intestinal cells (INT 407) and primary intestinal chick cells (PIC) was determined by quantitative real-time RT-PCR. The secretion of IL-8 protein by INT407 was measured using ELISA. Although C. jejuni and C. coli are considered to be harmless commensals in the gut of birds, the avian Campylobacter isolates investigated were able to induce the proinflammatory IL-8 in PIC as well as in INT407. In an in vitro system, C. jejuni as well as C. coli were able to induce IL-8 mRNA in PIC. Relation between the virulence properties like toxin production, the ability to invade and to survive in Caco-2 cells and the level of IL-8 mRNA produced by INT 407 and PIC after infection with Campylobacter strains was also investigated.

Links

PubMed Online version:10.1016/j.vetmic.2007.04.041

Keywords

Animals; Campylobacter/immunology; Campylobacter Infections/immunology; Campylobacter Infections/microbiology; Campylobacter Infections/veterinary; Campylobacter coli/immunology; Campylobacter jejuni/immunology; Cell Line; Chickens; DNA, Complementary/analysis; Enzyme-Linked Immunosorbent Assay/methods; Enzyme-Linked Immunosorbent Assay/veterinary; Humans; Immunohistochemistry/methods; Immunohistochemistry/veterinary; Interleukin-8/genetics; Interleukin-8/metabolism; Intestinal Mucosa/immunology; Intestinal Mucosa/microbiology; Poultry Diseases/immunology; Poultry Diseases/microbiology; RNA, Messenger/analysis; Reverse Transcriptase Polymerase Chain Reaction/methods; Reverse Transcriptase Polymerase Chain Reaction/veterinary; Specific Pathogen-Free Organisms; Virulence

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:A3DTN6

involved_in

GO:0042119: neutrophil activation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:A3DTN6

involved_in

GO:0010186: positive regulation of cellular defense response

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:A3DTN6

involved_in

GO:0030593: neutrophil chemotaxis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:A3DTN6

involved_in

GO:0042742: defense response to bacterium

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.