GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17537789

From GONUTS
Jump to: navigation, search
Citation

Park, JS, Valerius, MT and McMahon, AP (2007) Wnt/beta-catenin signaling regulates nephron induction during mouse kidney development. Development 134:2533-9

Abstract

Mammalian nephrons form as a result of a complex morphogenesis and patterning of a simple epithelial precursor, the renal vesicle. Renal vesicles are established from a mesenchymal progenitor population in response to inductive signals. Several lines of evidence support the sequential roles of two Wnt family members, Wnt9b and Wnt4, in renal vesicle induction. Using genetic approaches to specifically manipulate the activity of beta-catenin within the mesenchymal progenitor pool in mice, we investigated the potential role of the canonical Wnt pathway in these inductive events. Progenitor-cell-specific removal of beta-catenin activity completely blocked both the formation of renal vesicles and the expected molecular signature of an earlier inductive response. By contrast, activation of stabilized beta-catenin in the same cell population causes ectopic expression of mesenchymal induction markers in vitro and functionally replaces the requirement for Wnt9b and Wnt4 in their inductive roles in vivo. Thus, canonical Wnt signaling is both necessary and sufficient for initiating and maintaining inductive pathways mediated by Wnt9b and Wnt4. However, the failure of induced mesenchyme with high levels of beta-catenin activity to form epithelial structures suggests that modulating canonical signaling may be crucial for the cellular transition to the renal vesicle.

Links

PubMed Online version:10.1242/dev.006155

Keywords

Animals; Biological Markers; Cell Differentiation; Epithelial Cells/cytology; Epithelial Cells/metabolism; Gene Expression Regulation, Developmental; Kidney/cytology; Kidney/embryology; Kidney/metabolism; Mesenchymal Stem Cells/cytology; Mesenchymal Stem Cells/metabolism; Mesoderm/cytology; Mesoderm/metabolism; Mice; Mice, Transgenic; Signal Transduction; Wnt Proteins/genetics; Wnt Proteins/metabolism; beta Catenin/genetics; beta Catenin/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CTNB1

acts_upstream_of_or_within

GO:0072033: renal vesicle formation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1858008

P

Seeded From UniProt

complete

MOUSE:CTNB1

acts_upstream_of_or_within

GO:0072079: nephron tubule formation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1858008

P

Seeded From UniProt

complete

MOUSE:CTNB1

acts_upstream_of_or_within

GO:0001658: branching involved in ureteric bud morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1858008

P

Seeded From UniProt

complete

MOUSE:CTNB1

acts_upstream_of_or_within

GO:0001822: kidney development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1858008

P

Seeded From UniProt

complete

MOUSE:CTNB1

acts_upstream_of_or_within

GO:0003340: negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.