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PMID:17535250

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Citation

Kobayashi, T, Gengyo-Ando, K, Ishihara, T, Katsura, I and Mitani, S (2007) IFT-81 and IFT-74 are required for intraflagellar transport in C. elegans. Genes Cells 12:593-602

Abstract

Intraflagellar transport (IFT) is essential machinery for biogenesis and maintenance of cilia in many eukaryotic and prokaryotic cells. A large number of polypeptides are known to be involved in IFT, but the physiological role of each component is not fully elucidated. Here, we identified a C. elegans orthologue of a Chlamydomonas reinhardtii IFT component, IFT-81, and found that its loss-of-function mutants show an unusual behavioral property and small body size. IFT-81 is expressed in sensory neurons, and localized at the base of cilia. The similar phenotypes with ift-81 mutants were also observed in several IFT mutants, suggesting these defects are caused by inability of IFT. We also demonstrated that IFT-81 interacts and co-localizes with IFT-74, which is another putative component of IFT. The ift-74 loss-of-function mutants showed phenocopies with ift-81 mutants, suggesting IFT-81 and IFT-74 play comparable functions. Moreover, ift-81 and ift-74 mutants similarly exhibited weak anomalies in cilia formation and obvious disruptions of transport in mature cilia. Thus, we conclude that IFT-81 and IFT-74 coordinately act in IFT in C. elegans sensory cilia.

Links

PubMed Online version:10.1111/j.1365-2443.2007.01076.x

Keywords

Animals; Animals, Genetically Modified; Biological Transport, Active; Caenorhabditis elegans/genetics; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/physiology; Carrier Proteins/genetics; Carrier Proteins/physiology; Chemotaxis; Cilia/physiology; Flagella/physiology; Genes, Helminth; Mutation; Neurons, Afferent/physiology; Phenotype

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:A0A2C9C2L6

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00017973

F

Seeded From UniProt

complete

CAEEL:A0A2C9C2L6

located_in

GO:0097730: non-motile cilium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:A0A2C9C2L6

involved_in

GO:0006935: chemotaxis

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00251283

P

Seeded From UniProt

complete

CAEEL:A0A2C9C2L6

involved_in

GO:0050906: detection of stimulus involved in sensory perception

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00251283

P

Seeded From UniProt

complete

CAEEL:A0A2C9C2L6

involved_in

GO:0042073: intraciliary transport

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00251283

P

Seeded From UniProt

complete

CAEEL:A0A2C9C2L6

involved_in

GO:0035264: multicellular organism growth

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00251283

P

Seeded From UniProt

complete

CAEEL:A8DYR7

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00016005

F

Seeded From UniProt

complete

CAEEL:A8DYR7

located_in

GO:0097730: non-motile cilium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:A8DYR7

involved_in

GO:0006935: chemotaxis

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00251252

P

Seeded From UniProt

complete

CAEEL:A8DYR7

involved_in

GO:0050906: detection of stimulus involved in sensory perception

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00251252

P

Seeded From UniProt

complete

CAEEL:A8DYR7

involved_in

GO:0042073: intraciliary transport

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00251252

P

Seeded From UniProt

complete

CAEEL:A8DYR7

involved_in

GO:0035264: multicellular organism growth

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00251252

P

Seeded From UniProt

complete


See also

References

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