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PMID:17510633

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Citation

Mukhopadhyay, S, Lu, Y, Qin, H, Lanjuin, A, Shaham, S and Sengupta, P (2007) Distinct IFT mechanisms contribute to the generation of ciliary structural diversity in C. elegans. EMBO J. 26:2966-80

Abstract

Individual cell types can elaborate morphologically diverse cilia. Cilia are assembled via intraflagellar transport (IFT) of ciliary precursors; however, the mechanisms that generate ciliary diversity are unknown. Here, we examine IFT in the structurally distinct cilia of the ASH/ASI and the AWB chemosensory neurons in Caenorhabditis elegans, enabling us to compare IFT in specific cilia types. We show that unlike in the ASH/ASI cilia, the OSM-3 kinesin moves independently of the kinesin-II motor in the AWB cilia. Although OSM-3 is essential to extend the distal segments of the ASH/ASI cilia, it is not required to build the AWB distal segments. Mutations in the fkh-2 forkhead domain gene result in AWB-specific defects in ciliary morphology, and FKH-2 regulates kinesin-II subunit gene expression specifically in AWB. Our results suggest that cell-specific regulation of IFT contributes to the generation of ciliary diversity, and provide insights into the networks coupling the acquisition of ciliary specializations with other aspects of cell fate.

Links

PubMed PMC1894762 Online version:10.1038/sj.emboj.7601717

Keywords

Animals; Animals, Genetically Modified; Biological Transport; Caenorhabditis elegans/cytology; Caenorhabditis elegans Proteins/physiology; Cilia/ultrastructure; Kinesin/physiology; Microscopy, Electron; Mutation; Olfactory Receptor Neurons/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:Q22510

involved_in

GO:0016358: dendrite development

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00091967

P

Seeded From UniProt

complete

CAEEL:Q22510

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q22510

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00091967

F

Seeded From UniProt

complete

CAEEL:Q22510

involved_in

GO:0048665: neuron fate specification

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00091967

P

Seeded From UniProt

complete

CAEEL:Q22510

involved_in

GO:1905515: non-motile cilium assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00091967

P

Seeded From UniProt

complete

CAEEL:Q22510

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00091967

P

Seeded From UniProt

complete

CAEEL:Q95QA9

involved_in

GO:0016358: dendrite development

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00091967

P

Seeded From UniProt

complete

CAEEL:Q95QA9

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q95QA9

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00091967

F

Seeded From UniProt

complete

CAEEL:Q95QA9

involved_in

GO:0048665: neuron fate specification

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00091967

P

Seeded From UniProt

complete

CAEEL:Q95QA9

involved_in

GO:1905515: non-motile cilium assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00091967

P

Seeded From UniProt

complete

CAEEL:Q95QA9

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00091967

P

Seeded From UniProt

complete


See also

References

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