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PMID:17507402

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Citation

Lee, JD, Silva-Gagliardi, NF, Tepass, U, McGlade, CJ and Anderson, KV (2007) The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo. Development 134:2007-16

Abstract

During early mouse development, a single-layered epithelium is transformed into the three germ layers that are the basis of the embryonic body plan. Here we describe an ENU-induced mutation, limulus (lulu), which disrupts gastrulation and the organization of all three embryonic germ layers. Positional cloning and analysis of additional alleles show that lulu is a null allele of the FERM-domain gene erythrocyte protein band 4.1-like 5 (Epb4.1l5). During gastrulation, some cells in lulu mutants are trapped in the primitive streak at an intermediate stage of the epithelial-mesenchymal transition; as a result, the embryos have very little paraxial mesoderm. Epithelial layers of the later lulu embryo are also disrupted: definitive endoderm is specified but does not form a gut tube, and the neural plate is broad and forms ectopic folds rather than closing to make the neural tube. In contrast to zebrafish and Drosophila, in which orthologs of Epb4.1l5 control the apical localization and activity of Crumbs proteins, mouse Crumbs proteins are localized normally to the apical surface of the lulu mutant epiblast and neural plate. However, the defects in both the lulu primitive streak and neural plate are associated with disruption of the normal organization of the actin cytoskeleton. We propose that mouse Lulu (Epb4.1l5) helps anchor the actin-myosin contractile machinery to the membrane to allow the dynamic rearrangements of epithelia that mediate embryonic morphogenesis.

Links

PubMed Online version:10.1242/dev.000885

Keywords

Animals; Cell Differentiation/genetics; Cytoskeletal Proteins; Cytoskeleton/genetics; Cytoskeleton/physiology; Epithelium/embryology; Galactosides; Immunohistochemistry; In Situ Hybridization; Indoles; Membrane Proteins/genetics; Mesoderm/embryology; Mice; Morphogenesis/genetics; Morphogenesis/physiology; Mutation/genetics; Neural Plate/embryology; Primitive Streak/embryology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:E41L5

acts_upstream_of_or_within

GO:0030036: actin cytoskeleton organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:E41L5

acts_upstream_of_or_within

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3578147

P

  • occurs_in:(CL:0000222)
  • occurs_in:(EMAPA:16071)|occurs_in:(CL:0000222)
  • occurs_in:(EMAPA:16073)

Seeded From UniProt

complete

MOUSE:E41L5

acts_upstream_of_or_within

GO:0001839: neural plate morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3578147

P

Seeded From UniProt

complete

MOUSE:E41L5

involved_in

GO:0001837: epithelial to mesenchymal transition

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3578147

P

  • occurs_in:(EMAPA:16072)

Seeded From UniProt

complete

MOUSE:E41L5

acts_upstream_of_or_within

GO:0048319: axial mesoderm morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3578147

P

Seeded From UniProt

complete


See also

References

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