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PMID:17483425
| Citation |
Mathieu, J, Sung, HH, Pugieux, C, Soetaert, J and Rorth, P (2007) A sensitized PiggyBac-based screen for regulators of border cell migration in Drosophila. Genetics 176:1579-90 |
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| Abstract |
Migration of border cells during Drosophila melanogaster oogenesis is a good model system for investigating the genetic requirements for cell migration in vivo. We present a sensitized loss-of-function screen used to identify new genes required in border cells for their migration. Chromosomes bearing FRTs on all four major autosomal arms were mutagenized by insertions of the transposable element PiggyBac, allowing multiple parallel clonal screens and easy identification of the mutated gene. For border cells, we analyzed homozygous mutant clones positively marked with lacZ and sensitized by expression of dominant-negative PVR, the guidance receptor. We identified new alleles of genes already known to be required for border cell migration, including aop/yan, DIAP1, and taiman as well as a conserved Slbo-regulated enhancer downstream of shg/DE-cadherin. Mutations in genes not previously described to be required in border cells were also uncovered: hrp48, vir, rme-8, kismet, and puckered. puckered was unique in that the migration defects were observed only when PVR signaling was reduced. We present evidence that an excess of JNK signaling is deleterious for migration in the absence of PVR activity at least in part through Fos transcriptional activity and possibly through antagonistic effects on DIAP1. |
| Links |
PubMed PMC1931525 Online version:10.1534/genetics.107.071282 |
| Keywords |
Animals; Cell Movement; DNA Transposable Elements; Drosophila Proteins/antagonists & inhibitors; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Inhibitor of Apoptosis Proteins/antagonists & inhibitors; JNK Mitogen-Activated Protein Kinases/metabolism; Mutation; Oogenesis; Receptor Protein-Tyrosine Kinases/metabolism; Signal Transduction; Transcription, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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