GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17464331

From GONUTS
Jump to: navigation, search
Citation

Nakade, K, Pan, J, Yoshiki, A, Ugai, H, Kimura, M, Liu, B, Li, H, Obata, Y, Iwama, M, Itohara, S, Murata, T and Yokoyama, KK (2007) JDP2 suppresses adipocyte differentiation by regulating histone acetylation. Cell Death Differ. 14:1398-405

Abstract

Among the events that control cellular differentiation, the acetylation of histones plays a critical role in the regulation of transcription and the modification of chromatin. Jun dimerization protein 2 (JDP2), a member of the AP-1 family, is an inhibitor of such acetylation and contributes to the maintenance of chromatin structure. In an examination of Jdp2 'knock-out' (KO) mice, we observed elevated numbers of white adipocytes and significant accumulation of lipid in the adipose tissue in sections of scapulae. In addition, mouse embryo fibroblasts (MEFs) from Jdp2 KO mice were more susceptible to adipocyte differentiation in response to hormonal induction and members of the CCAAT/enhancer-binding proteins (C/EBP) gene family were expressed at levels higher than MEFs from wild-type mice. Furthermore, JDP2 inhibited both the acetylation of histone H3 in the promoter of the gene for C/EBPdelta and transcription from this promoter. Our data indicate that JDP2 plays a key role as a repressor of adipocyte differentiation by regulating the expression of the gene for C/EBPdelta via inhibition of histone acetylation.

Links

PubMed Online version:10.1038/sj.cdd.4402129

Keywords

3T3-L1 Cells; Acetylation; Adipocytes/cytology; Adipocytes/metabolism; Adipogenesis/genetics; Adipogenesis/physiology; Animals; Base Sequence; CCAAT-Enhancer-Binding Protein-delta/genetics; Cell Differentiation/physiology; DNA Primers/genetics; Female; Fibroblasts/cytology; Fibroblasts/metabolism; Gene Targeting; Histones/chemistry; Histones/metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Mutation; Pregnancy; Promoter Regions, Genetic; Repressor Proteins/genetics; Repressor Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:HDAC3

involved_in

GO:0016575: histone deacetylation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1932093

P

Seeded From UniProt

complete

MOUSE:JDP2

enables

GO:0003682: chromatin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:JDP2

involved_in

GO:0045444: fat cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:JDP2

involved_in

GO:0045599: negative regulation of fat cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:JDP2

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:JDP2

involved_in

GO:0031065: positive regulation of histone deacetylation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1343091

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.