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PMID:17464325

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Citation

Baum, JS, Arama, E, Steller, H and McCall, K (2007) The Drosophila caspases Strica and Dronc function redundantly in programmed cell death during oogenesis. Cell Death Differ. 14:1508-17

Abstract

Programmed cell death (PCD) in the Drosophila ovary occurs either during mid-oogenesis, resulting in degeneration of the entire egg chamber or during late oogenesis, to facilitate the development of the oocyte. PCD during oogenesis is regulated by mechanisms different from those that control cell death in other Drosophila tissues. We have analyzed the role of caspases in PCD of the female germline by examining caspase mutants and overexpressing caspase inhibitors. Imprecise P-element excision was used to generate mutants of the initiator caspase strica. While null mutants of strica or another initiator caspase, dronc, display no ovary phenotype, we find that strica exhibits redundancy with dronc, during both mid- and late oogenesis. Ovaries of double mutants contain defective mid-stage egg chambers similar to those reported previously in dcp-1 mutants, and mature egg chambers with persisting nurse cell nuclei. In addition, the effector caspases drice and dcp-1 also display redundant functions during late oogenesis, resulting in persisting nurse cell nuclei. These findings indicate that caspases are required for nurse cell death during mid-oogenesis, and participate in developmental nurse cell death during late oogenesis. This reveals a novel pathway of cell death in the ovary that utilizes strica, dronc, dcp-1 and drice, and importantly illustrates strong redundancy among the caspases.

Links

PubMed Online version:10.1038/sj.cdd.4402155

Keywords

Animals; Animals, Genetically Modified; Apoptosis/genetics; Apoptosis/physiology; Base Sequence; Caspases/genetics; Caspases/physiology; DNA, Complementary/genetics; Drosophila/cytology; Drosophila/enzymology; Drosophila/genetics; Drosophila/growth & development; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Female; Gene Expression Regulation, Developmental; Genes, Insect; Mutation; Oogenesis/genetics; Oogenesis/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:DRICE

involved_in

GO:0045476: nurse cell apoptotic process

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010501

P

Seeded From UniProt

complete

DROME:CASP1

involved_in

GO:0045476: nurse cell apoptotic process

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0019972

P

Seeded From UniProt

complete

DROME:DIAP1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:DIAP1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q7KHK9

involved_in

GO:0045476: nurse cell apoptotic process

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0026404

P

Seeded From UniProt

complete

DROME:DRONC

involved_in

GO:0045476: nurse cell apoptotic process

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0033051

P

Seeded From UniProt

complete

DROME:IAP1

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:IAP1

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ICENC

involved_in

GO:0045476: nurse cell apoptotic process

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0033051

P

Seeded From UniProt

complete

See also

References

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