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PMID:17446352

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Citation

Aravin, AA, Sachidanandam, R, Girard, A, Fejes-Toth, K and Hannon, GJ (2007) Developmentally regulated piRNA clusters implicate MILI in transposon control. Science 316:744-7

Abstract

Nearly half of the mammalian genome is composed of repeated sequences. In Drosophila, Piwi proteins exert control over transposons. However, mammalian Piwi proteins, MIWI and MILI, partner with Piwi-interacting RNAs (piRNAs) that are depleted of repeat sequences, which raises questions about a role for mammalian Piwi's in transposon control. A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. We also find evidence of an adaptive amplification loop in which MILI catalyzes the formation of piRNA 5' ends. Mili mutants derepress LINE-1 (L1) and intracisternal A particle and lose DNA methylation of L1 elements, demonstrating an evolutionarily conserved role for PIWI proteins in transposon suppression.

Links

PubMed Online version:10.1126/science.1142612

Keywords

3' Untranslated Regions; Animals; Argonaute Proteins; Cluster Analysis; Computational Biology; DNA Methylation; Genes, Intracisternal A-Particle; Long Interspersed Nucleotide Elements; Male; Meiosis; Mice; Mutation; Proteins/metabolism; RNA, Antisense/genetics; RNA, Antisense/metabolism; RNA, Untranslated/genetics; RNA, Untranslated/metabolism; Retroelements; Reverse Transcriptase Polymerase Chain Reaction; Short Interspersed Nucleotide Elements; Spermatocytes/cytology; Spermatocytes/metabolism; Spermatogenesis; Suppression, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:PIWL2

involved_in

GO:0000966: RNA 5'-end processing

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:PIWL2

involved_in

GO:0031047: gene silencing by RNA

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:PIWL2

enables

GO:0034584: piRNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:PIWL2

involved_in

GO:0034587: piRNA metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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