PMID:17426032

Citation	Rasbery, JM, Shan, H, LeClair, RJ, Norman, M, Matsuda, SP and Bartel, B (2007) Arabidopsis thaliana squalene epoxidase 1 is essential for root and seed development. J. Biol. Chem. 282:17002-13
Abstract	Squalene epoxidase converts squalene into oxidosqualene, the precursor of all known angiosperm cyclic triterpenoids, which include membrane sterols, brassinosteroid phytohormones, and non-steroidal triterpenoids. In this work, we have identified six putative Arabidopsis squalene epoxidase (SQE) enzymes and used heterologous expression in yeast to demonstrate that three of these enzymes, SQE1, SQE2, and SQE3, can epoxidize squalene. We isolated and characterized Arabidopsis sqe1 mutants and discovered severe developmental defects, including reduced root and hypocotyl elongation. Adult sqe1-3 and sqe1-4 plants have diminished stature and produce inviable seeds. The sqe1-3 mutant accumulates squalene, consistent with a block in the triterpenoid biosynthetic pathway. Therefore, SQE1 function is necessary for normal plant development, and the five SQE-like genes remaining in this mutant are not fully redundant with SQE1.
Links	PubMed Online version:10.1074/jbc.M611831200
Keywords	Amino Acid Sequence; Arabidopsis/embryology; Arabidopsis/enzymology; Arabidopsis/growth & development; Base Sequence; DNA Primers; Genes, Plant; Molecular Sequence Data; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Seeds/growth & development; Sequence Homology, Amino Acid; Squalene Monooxygenase/chemistry; Squalene Monooxygenase/genetics; Squalene Monooxygenase/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:ERG16	acts_upstream_of_or_within	GO:0016126: sterol biosynthetic process	ECO:0000304: author statement supported by traceable reference used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.