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PMID:17409354

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Citation

Jara, M, Vivancos, AP, Calvo, IA, Moldón, A, Sansó, M and Hidalgo, E (2007) The peroxiredoxin Tpx1 is essential as a H2O2 scavenger during aerobic growth in fission yeast. Mol. Biol. Cell 18:2288-95

Abstract

Peroxiredoxins are known to interact with hydrogen peroxide (H(2)O(2)) and to participate in oxidant scavenging, redox signal transduction, and heat-shock responses. The two-cysteine peroxiredoxin Tpx1 of Schizosaccharomyces pombe has been characterized as the H(2)O(2) sensor that transduces the redox signal to the transcription factor Pap1. Here, we show that Tpx1 is essential for aerobic, but not anaerobic, growth. We demonstrate that Tpx1 has an exquisite sensitivity for its substrate, which explains its participation in maintaining low steady-state levels of H(2)O(2). We also show in vitro and in vivo that inactivation of Tpx1 by oxidation of its catalytic cysteine to a sulfinic acid is always preceded by a sulfinic acid form in a covalently linked dimer, which may be important for understanding the kinetics of Tpx1 inactivation. Furthermore, we provide evidence that a strain expressing Tpx1.C169S, lacking the resolving cysteine, can sustain aerobic growth, and we show that small reductants can modulate the activity of the mutant protein in vitro, probably by supplying a thiol group to substitute for cysteine 169.

Links

PubMed PMC1877099 Online version:10.1091/mbc.E06-11-1039

Keywords

Aerobiosis/physiology; Animals; Cysteine/metabolism; Hydrogen Peroxide/metabolism; Oxidants/metabolism; Oxidation-Reduction; Peroxidases/genetics; Peroxidases/metabolism; Peroxiredoxins; Protein Isoforms/genetics; Protein Isoforms/metabolism; Schizosaccharomyces/physiology; Schizosaccharomyces pombe Proteins; Substrate Specificity; Sulfinic Acids/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:TRX1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPCC576.03c

F

Seeded From UniProt

complete

SCHPO:TRX1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPBC3F6.03

F

Seeded From UniProt

complete

SCHPO:TRX1

enables

GO:0016209: antioxidant activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:TRX1

enables

GO:0015035: protein-disulfide reductase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:TPX1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPAC7D4.07c

F

Seeded From UniProt

complete

SCHPO:TPX1

enables

GO:0004601: peroxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:TPX1

involved_in

GO:0045454: cell redox homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:TPX1

enables

GO:0051920: peroxiredoxin activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:SODC

involved_in

GO:0019430: removal of superoxide radicals

ECO:0000305: curator inference used in manual assertion

GO:0004784

P

Seeded From UniProt

complete

SCHPO:SODC

enables

GO:0004784: superoxide dismutase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:SODC

involved_in

GO:0045454: cell redox homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:TRXB

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPAC7D4.07c

F

Seeded From UniProt

complete

SCHPO:TRXB

enables

GO:0004791: thioredoxin-disulfide reductase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:TSA1

enables

GO:0004601: peroxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:TSA1

involved_in

GO:0045454: cell redox homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:TSA1

enables

GO:0051920: peroxiredoxin activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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