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PMID:17363962

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Citation

Juhász, G, Puskás, LG, Komonyi, O, Erdi, B, Maróy, P, Neufeld, TP and Sass, M (2007) Gene expression profiling identifies FKBP39 as an inhibitor of autophagy in larval Drosophila fat body. Cell Death Differ. 14:1181-90

Abstract

In Drosophila, the fat body undergoes a massive burst of autophagy at the end of larval development in preparation for the pupal transition. To identify genes involved in this process, we carried out a microarray analysis. We found that mRNA levels of the homologs of Atg8, the coat protein of early autophagic structures, and lysosomal hydrolases were upregulated, consistent with previous results. Genes encoding mitochondrial proteins and many chaperones were downregulated, including the inhibitor of eIF2alpha kinases and the peptidyl-prolyl cis-trans isomerase FK506-binding protein of 39 kDa (FKBP39). Genetic manipulation of FKBP39 expression had a significant effect on autophagy, potentially through modulation of the transcription factor Foxo. Accordingly, we found that Foxo mutants cannot properly undergo autophagy in response to starvation, and that overexpression of Foxo induces autophagy.

Links

PubMed PMC2084463 Online version:10.1038/sj.cdd.4402123

Keywords

Animals; Autophagy/genetics; Autophagy/physiology; Drosophila/genetics; Drosophila/growth & development; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Fat Body/metabolism; Forkhead Transcription Factors/genetics; Forkhead Transcription Factors/physiology; Gene Expression Profiling; Gene Expression Regulation, Developmental; Larva/genetics; Mutation; Oligonucleotide Array Sequence Analysis; PTEN Phosphohydrolase/genetics; PTEN Phosphohydrolase/physiology; Reverse Transcriptase Polymerase Chain Reaction; Tacrolimus Binding Proteins/genetics; Tacrolimus Binding Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:FKB39

involved_in

GO:0016242: negative regulation of macroautophagy

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:FKB39

involved_in

GO:0010507: negative regulation of autophagy

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:FOXO

involved_in

GO:0016241: regulation of macroautophagy

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:FOXO

involved_in

GO:0010506: regulation of autophagy

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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