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PMID:17362950

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Citation

McFarland, DC, Velleman, SG, Pesall, JE and Liu, C (2007) The role of myostatin in chicken (Gallus domesticus) myogenic satellite cell proliferation and differentiation. Gen. Comp. Endocrinol. 151:351-7

Abstract

Myostatin (GDF-8) inhibits the activation, proliferation, and differentiation of myogenic satellite cells. The relative importance of this growth factor is demonstrated in myostatin-null mice and cattle possessing defective myostatin genes. These defects result in greatly enhanced musculature. In the present study, the effect of myostatin on chicken myogenic satellite cells derived from two different skeletal muscles was studied. The effect of anti-myostatin antibodies on cellular responses was also examined. Satellite cells isolated from the pectoralis major (PM) muscles were more responsive to the proliferation depressing effects of myostatin compared to cells from the biceps femoris (BF; P <or= 0.05). Myostatin inhibited differentiation of satellite cells derived from the PM muscle (P <or=0.05), but had no effect on cells from the BF (P >or=0.05). Myostatin administered to proliferating cells depressed the synthesis of decorin (P <or= 0.05), an extracellular matrix proteoglycan. However, in differentiating cultures, only BF cells expressed lower levels of decorin (P <or= 0.05). Decorin expression in PM cells was unchanged (P>or= 0.05). Administration of anti-myostatin antibodies to proliferating cultures increased cell proliferation by 6-7% over 3 days. There was no effect on differentiation of either PM or BF cells. The present study demonstrates that there are differences in the responsiveness to myostatin of chicken satellite cells derived from different muscles. Evidence is also given to support the role of endogenous myostatin in autocrine regulation of muscle growth.

Links

PubMed Online version:10.1016/j.ygcen.2007.02.006

Keywords

Animals; Cell Differentiation/drug effects; Cell Proliferation/drug effects; Cells, Cultured; Chickens/physiology; Female; Myostatin; Pectoralis Muscles/cytology; Pectoralis Muscles/drug effects; Pectoralis Muscles/growth & development; Quadriceps Muscle/cytology; Quadriceps Muscle/drug effects; Quadriceps Muscle/growth & development; Satellite Cells, Skeletal Muscle/cytology; Satellite Cells, Skeletal Muscle/drug effects; Transforming Growth Factor beta/pharmacology; Transforming Growth Factor beta/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:D1LWV1

involved_in

GO:0033002: muscle cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(CL:0000594)
  • occurs_in:(UBERON:0002381)
  • occurs_in:(UBERON:0001374)

Seeded From UniProt

complete

CHICK:D1LWV1

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:D1LWV2

involved_in

GO:0033002: muscle cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(CL:0000594)
  • occurs_in:(UBERON:0002381)
  • occurs_in:(UBERON:0001374)

Seeded From UniProt

complete

CHICK:D1LWV2

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:GDF8

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q06BS3

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:GDF8

part_of

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:GDF8

involved_in

GO:0033002: muscle cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(CL:0000594)
  • occurs_in:(UBERON:0002381)
  • occurs_in:(UBERON:0001374)

Seeded From UniProt

complete

CHICK:Q06BS3

part_of

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q06BS3

involved_in

GO:0033002: muscle cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(CL:0000594)
  • occurs_in:(UBERON:0002381)
  • occurs_in:(UBERON:0001374)

Seeded From UniProt

complete

Notes

See also

References

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