GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17350955

From GONUTS
Jump to: navigation, search
Citation

Tanaka, SY, Narita, S and Tokuda, H (2007) Characterization of the Pseudomonas aeruginosa Lol system as a lipoprotein sorting mechanism. J. Biol. Chem. 282:13379-84

Abstract

Escherichia coli lipoproteins are localized to either the inner or the outer membrane depending on the residue that is present next to the N-terminal acylated Cys. Asp at position 2 causes the retention of lipoproteins in the inner membrane. In contrast, the accompanying study (9) revealed that the residues at positions 3 and 4 determine the membrane specificity of lipoproteins in Pseudomonas aeruginosa. Since the five Lol proteins involved in the sorting of E. coli lipoproteins are conserved in P. aeruginosa, we examined whether or not the Lol proteins of P. aeruginosa are also involved in lipoprotein sorting but utilize different signals. The genes encoding LolCDE, LolA, and LolB homologues were cloned and expressed. The LolCDE homologue thus purified was reconstituted into proteoliposomes with lipoproteins. When incubated in the presence of ATP and a LolA homologue, the reconstituted LolCDE homologue released lipoproteins, leading to the formation of a LolA-lipoprotein complex. Lipoproteins were then incorporated into the outer membrane depending on a LolB homologue. As revealed in vivo, lipoproteins with Lys and Ser at positions 3 and 4, respectively, remained in proteoliposomes. On the other hand, E. coli LolCDE released lipoproteins with this signal and transferred them to LolA of not only E. coli but also P. aeruginosa. These results indicate that Lol proteins are responsible for the sorting of lipoproteins to the outer membrane of P. aeruginosa, as in the case of E. coli, but respond differently to inner membrane retention signals.

Links

PubMed Online version:10.1074/jbc.M611840200

Keywords

Bacterial Outer Membrane Proteins/chemistry; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Membrane/chemistry; Cell Membrane/genetics; Cell Membrane/metabolism; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Liposomes/chemistry; Protein Transport/physiology; Pseudomonas aeruginosa/chemistry; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/metabolism; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEAE:LOLB

acts_upstream_of_or_within

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:Q9HZL6

acts_upstream_of_or_within

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LOLD

acts_upstream_of_or_within

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:Q9HZL8

acts_upstream_of_or_within

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LOLA

acts_upstream_of_or_within

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LOLD

GO:0089705: protein localization to outer membrane

ECO:0000314:

P

Fig3A

complete
CACAO 10461

PSEAE:LOLD

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314:

P

Fig3A

complete
CACAO 10462

PSEAE:LOLD

involved_in

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LOLD

involved_in

GO:0089705: protein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LOLA

involved_in

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LOLA

GO:0089705: protein localization to outer membrane

ECO:0000314:

P

Fig3, A&B

complete
CACAO 10463

PSEAE:LOLA

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314:

P

Fig3 A&B

complete
CACAO 10464

PSEAE:LOLA

GO:0072323: chaperone-mediated protein transport across periplasmic space

ECO:0000314:

P

Fig3 A&B

complete
CACAO 10465

PSEAE:LOLA

involved_in

GO:0072323: chaperone-mediated protein transport across periplasmic space

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:Q9HZL8

involved_in

GO:0089705: protein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:Q9HZL8

involved_in

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:Q9HZL8

GO:0089705: protein localization to outer membrane

ECO:0000314:

P

Fig3A

complete
CACAO 10466

PSEAE:Q9HZL8

GO:0042953: lipoprotein transport

ECO:0000314:

P

Fig3A

complete
CACAO 10467

PSEAE:Q9HZL8

involved_in

GO:0042953: lipoprotein transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:Q9HZL6

GO:0089705: protein localization to outer membrane

ECO:0000314:

P

Fig3A

complete
CACAO 10468

PSEAE:Q9HZL6

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314:

P

Fig3A

complete
CACAO 10469

PSEAE:Q9HZL6

involved_in

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LOLB

involved_in

GO:0089705: protein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LOLB

involved_in

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LOLB

GO:0089705: protein localization to outer membrane

ECO:0000314:

P

Fig3B

complete
CACAO 10470

PSEAE:LOLB

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314:

P

Fig3B

complete
CACAO 10471

Notes

See also

References

See Help:References for how to manage references in GONUTS.