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PMID:17332324

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Citation

Horikawa, T, Yang, J, Kondo, S, Yoshizaki, T, Joab, I, Furukawa, M and Pagano, JS (2007) Twist and epithelial-mesenchymal transition are induced by the EBV oncoprotein latent membrane protein 1 and are associated with metastatic nasopharyngeal carcinoma. Cancer Res. 67:1970-8

Abstract

Nasopharyngeal carcinoma (NPC), an EBV-associated malignancy, is highly metastatic compared with other head and neck tumors, perhaps because of its viral link. Here, we show that the principal EBV oncoprotein, latent membrane protein 1 (LMP1), induces epithelial-mesenchymal transition (EMT) via Twist, a master transcriptional regulator in embryogenesis and newly implicated in metastasis, which, in turn, are likely to contribute to the highly metastatic character of NPC. LMP1 could induce EMT and its associated cell motility and invasiveness in a cell culture model, whereas expression of Twist small interfering RNA reversed LMP1-induced EMT. In diverse EBV-infected cell lines, expression of Twist correlates with expression of LMP1. Dominant-negative LMP1 could suppress Twist expression in EBV-positive cells, whereas LMP1 could induce Twist in EBV-negative nasopharyngeal cells. LMP1 signals through the nuclear factor-kappaB pathway, and an IkappaB superrepressor inhibited induction of Twist by LMP1. Finally, in human NPC tissues, expression of Twist and LMP1 is directly correlated and expression of Twist is associated with metastasis clinically. These results suggest that induction of Twist by a human viral oncoprotein LMP1 directly contributes to the metastatic nature of NPC.

Links

PubMed Online version:10.1158/0008-5472.CAN-06-3933

Keywords

Animals; Carcinoma/metabolism; Carcinoma/pathology; Carcinoma/virology; Cell Movement; Cell Transformation, Neoplastic/metabolism; Dogs; Epithelial Cells/cytology; Epithelial Cells/metabolism; Epstein-Barr Virus Infections/metabolism; HeLa Cells; Herpesvirus 4, Human/physiology; Humans; Mesenchymal Stem Cells/cytology; Mesenchymal Stem Cells/metabolism; Nasopharyngeal Neoplasms/metabolism; Nasopharyngeal Neoplasms/pathology; Nasopharyngeal Neoplasms/virology; Neoplasm Invasiveness; Neoplasm Metastasis/pathology; Nuclear Proteins/metabolism; Nuclear Proteins/physiology; Tumor Cells, Cultured; Twist Transcription Factor/metabolism; Twist Transcription Factor/physiology; Viral Matrix Proteins/physiology; Virus Latency

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:TWST1

involved_in

GO:0010718: positive regulation of epithelial to mesenchymal transition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:TWST1

involved_in

GO:2000147: positive regulation of cell motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:TWST1

involved_in

GO:2000147: positive regulation of cell motility

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete


See also

References

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