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PMID:17320070

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Citation

Yonekura, S, Xu, L, Ting, CY and Lee, CH (2007) Adhesive but not signaling activity of Drosophila N-cadherin is essential for target selection of photoreceptor afferents. Dev. Biol. 304:759-70

Abstract

Drosophila N-cadherin (CadN) is an evolutionarily conserved, atypical classical cadherin, which has a large complex extracellular domain and a catenin-binding cytoplasmic domain. We have previously shown that CadN regulates target selection of R7 photoreceptor axons. To determine the functional domains of CadN, we conducted a structure-function analysis focusing on its in vitro adhesive activity and in vivo function in R7 growth cones. We found that the cytoplasmic domain of CadN is largely dispensable for the targeting of R7 growth cones, and it is not essential for mediating homophilic interaction in cultured cells. Instead, the cytoplasmic domain of CadN is required for maintaining proper growth cone morphology. Domain swapping with the extracellular domain of CadN2, a related but non-adhesive cadherin, revealed that the CadN extracellular domain is required for both adhesive activity and R7 targeting. Using a target-mosaic system, we generated CadN mutant clones in the optic lobe and examined the target-selection of genetically wild-type R7 growth cones to CadN mutant target neurons. We found that CadN, but neither LAR nor Liprin-alpha, is required in the medulla neurons for R7 growth cones to select the correct medulla layer. Together, these data suggest that CadN mediates homophilic adhesive interactions between R7 growth cones and medulla neurons to regulate layer-specific target selection.

Links

PubMed PMC1959568 Online version:10.1016/j.ydbio.2007.01.030

Keywords

Animals; Cadherins/genetics; Cadherins/physiology; Cell Adhesion/physiology; Cells, Cultured; Drosophila/cytology; Drosophila/genetics; Drosophila/physiology; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Growth Cones/physiology; Mutation; Photoreceptor Cells, Invertebrate/physiology; Protein Isoforms/genetics; Protein Isoforms/physiology; Protein Structure, Tertiary; Signal Transduction/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:CADN

involved_in

GO:0007156: homophilic cell adhesion via plasma membrane adhesion molecules

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CADN

involved_in

GO:0045467: R7 cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CADN

involved_in

GO:0045463: R8 cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CADN

involved_in

GO:0044331: cell-cell adhesion mediated by cadherin

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CADN2

involved_in

GO:0045467: R7 cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CADN2

involved_in

GO:0045463: R8 cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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