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PMID:17307925

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Citation

Fujii, H, Verslues, PE and Zhu, JK (2007) Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis. Plant Cell 19:485-94

Abstract

Abscisic acid (ABA) is an important phytohormone regulating various plant processes, including seed germination. Although phosphorylation has been suggested to be important, the protein kinases required for ABA signaling during seed germination and seedling growth remain elusive. Here, we show that two protein kinases, SNF1-RELATED PROTEIN KINASE2.2 (SnRK2.2) and SnRK2.3, control responses to ABA in seed germination, dormancy, and seedling growth in Arabidopsis thaliana. A snrk2.2 snrk2.3 double mutant, but not snrk2.2 or snrk2.3 single mutants, showed strong ABA-insensitive phenotypes in seed germination and root growth inhibition. Changes in seed dormancy and ABA-induced Pro accumulation consistent with ABA insensitivity were also observed. The snrk2.2 snrk2.3 double mutant had a greatly reduced level of a 42-kD kinase activity capable of phosphorylating peptides from ABF (for ABA Response Element Binding Factor) transcription factors. ABA-induced expression of several genes whose promoters contain an ABA response element (ABRE) was reduced in snrk2.2 snrk2.3, suggesting that the mechanism of SnRK2.2 and SnRK2.3 action in ABA signaling involves the activation of ABRE-driven gene expression through the phosphorylation of ABFs. Together, these results demonstrate that SnRK2.2 and SnRK2.3 are redundant but key protein kinases that mediate a major part of ABA signaling in Arabidopsis.

Links

PubMed PMC1867333 Online version:10.1105/tpc.106.048538

Keywords

Abscisic Acid/metabolism; Arabidopsis/anatomy & histology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Gene Expression Regulation, Plant; Germination; Isoenzymes/genetics; Isoenzymes/metabolism; Molecular Sequence Data; Phosphorylation; Plant Growth Regulators/metabolism; Plant Leaves/metabolism; Plant Roots/anatomy & histology; Plant Roots/growth & development; Plants, Genetically Modified; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Seeds/physiology; Signal Transduction/physiology; Transcription Factors/genetics; Transcription Factors/metabolism; Water/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4J0N1

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4J0N1

enables

GO:0004672: protein kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:SRK2I

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SRK2I

enables

GO:0004672: protein kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

See also

References

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