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PMID:17303075

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Citation

Amorim, BR, Okamura, H, Yoshida, K, Qiu, L, Morimoto, H and Haneji, T (2007) The transcriptional factor Osterix directly interacts with RNA helicase A. Biochem. Biophys. Res. Commun. 355:347-51

Abstract

Osterix is an osteoblast-specific transcriptional factor, required for bone formation and osteoblast differentiation. Here, we identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among the candidates, RNA helicase A was identified to interact with Osterix. To determine the interaction of Osterix with RNA helicase A, immunoprecipitation assay was performed. Western analysis confirmed the association between Osterix and RNA helicase A. Immunocytochemical analysis also showed that Osterix and RNA helicase A were co-localized in HEK 293 cells. Our data suggest that RNA helicase A might be a component of Osterix regulation.

Links

PubMed Online version:10.1016/j.bbrc.2007.01.150

Keywords

Amino Acid Sequence; Cell Line; Humans; Immunoprecipitation; Molecular Sequence Data; Protein Binding; RNA Helicases/chemistry; RNA Helicases/metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:SP7

enables

GO:0017151: DEAD/H-box RNA helicase binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q8VI67

F

Seeded From UniProt

complete

MOUSE:SP7

enables

GO:0017151: DEAD/H-box RNA helicase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q08211

F

Seeded From UniProt

complete

HUMAN:DHX9

enables

GO:0001085: RNA polymerase II transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8VI67

F

Seeded From UniProt

complete


See also

References

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