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PMID:17272294

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Citation

Weinert, BT and Rio, DC (2007) DNA strand displacement, strand annealing and strand swapping by the Drosophila Bloom's syndrome helicase. Nucleic Acids Res. 35:1367-76

Abstract

Genetic analysis of the Drosophila Bloom's syndrome helicase homolog (mus309/DmBLM) indicates that DmBLM is required for the synthesis-dependent strand annealing (SDSA) pathway of homologous recombination. Here we report the first biochemical study of DmBLM. Recombinant, epitope-tagged DmBLM was expressed in Drosophila cell culture and highly purified protein was prepared from nuclear extracts. Purified DmBLM exists exclusively as a high molecular weight ( approximately 1.17 MDa) species, is a DNA-dependent ATPase, has 3'-->5' DNA helicase activity, prefers forked substrate DNAs and anneals complementary DNAs. High-affinity DNA binding is ATP-dependent and low-affinity ATP-independent interactions contribute to forked substrate DNA binding and drive strand annealing. DmBLM combines DNA strand displacement with DNA strand annealing to catalyze the displacement of one DNA strand while annealing a second complementary DNA strand.

Links

PubMed PMC1849897 Online version:10.1093/nar/gkl831

Keywords

Animals; DNA/metabolism; DNA Helicases/isolation & purification; DNA Helicases/metabolism; DNA, Single-Stranded/metabolism; Drosophila Proteins/isolation & purification; Drosophila Proteins/metabolism; Drosophila melanogaster/enzymology; Recombination, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:BLM

involved_in

GO:0000732: strand displacement

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:BLM

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:BLM

involved_in

GO:0032508: DNA duplex unwinding

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:BLM

enables

GO:0000403: Y-form DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:BLM

enables

GO:0043138: 3'-5' DNA helicase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:BLM

enables

GO:1990814: DNA/DNA annealing activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

See also

References

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