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PMID:17267557

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Citation

Gorczyca, D, Ashley, J, Speese, S, Gherbesi, N, Thomas, U, Gundelfinger, E, Gramates, LS and Budnik, V (2007) Postsynaptic membrane addition depends on the Discs-Large-interacting t-SNARE Gtaxin. J. Neurosci. 27:1033-44

Abstract

Targeted membrane addition is a hallmark of many cellular functions. In the nervous system, modification of synaptic membrane size has a major impact on synaptic function. However, because of the complex shape of neurons and the need to target membrane addition to very small and polarized synaptic compartments, this process is poorly understood. Here, we show that Gtaxin (GTX), a Drosophila t-SNARE (target-soluble N-ethylmaleimide-sensitive factor attachment protein receptor), is required for expansion of postsynaptic membranes during new synapse formation. Mutations in gtx lead to drastic reductions in postsynaptic membrane surface, whereas gtx upregulation results in the formation of complex membrane structures at ectopic sites. Postsynaptic GTX activity depends on its direct interaction with Discs-Large (DLG), a multidomain scaffolding protein of the PSD-95 (postsynaptic density protein-95) family with key roles in cell polarity and formation of cellular junctions as well as synaptic protein anchoring and trafficking. We show that DLG selectively determines the postsynaptic distribution of GTX to type I, but not to type II or type III boutons on the same cell, thereby defining sites of membrane addition to this unique set of glutamatergic synapses. We provide a mechanistic explanation for selective targeted membrane expansion at specific synaptic junctions.

Links

PubMed Online version:10.1523/JNEUROSCI.3160-06.2007

Keywords

Amino Acid Sequence; Animals; Drosophila; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila Proteins/physiology; Drug Resistance; Molecular Sequence Data; Presynaptic Terminals/metabolism; Presynaptic Terminals/physiology; Presynaptic Terminals/ultrastructure; SNARE Proteins/genetics; SNARE Proteins/metabolism; SNARE Proteins/physiology; Synaptic Membranes/physiology; Synaptic Membranes/ultrastructure; Tumor Suppressor Proteins/genetics; Tumor Suppressor Proteins/metabolism; Tumor Suppressor Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q9VC58

located_in

GO:0031594: neuromuscular junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VC58

involved_in

GO:0007528: neuromuscular junction development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VC58

involved_in

GO:0007274: neuromuscular synaptic transmission

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VC58

located_in

GO:0005938: cell cortex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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