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PMID:17264123

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Citation

Hamard, PJ, Boyer-Guittaut, M, Camuzeaux, B, Dujardin, D, Hauss, C, Oelgeschläger, T, Vigneron, M, Kedinger, C and Chatton, B (2007) Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity. Nucleic Acids Res. 35:1134-44

Abstract

Over the past few years, small ubiquitin-like modifier (SUMO) modification has emerged as an important regulator of diverse pathways and activities including protein localization and transcriptional regulation. We identified a consensus sumoylation motif (IKEE), located within the N-terminal activation domain of the ATF7 transcription factor and thus investigated the role of this modification. ATF7 is a ubiquitously expressed transcription factor, homologous to ATF2, that binds to CRE elements within specific promoters. This protein is able to heterodimerize with Jun or Fos proteins and its transcriptional activity is mediated by interaction with TAF12, a subunit of the general transcription factor TFIID. In the present article, we demonstrate that ATF7 is sumoylated in vitro (using RanBP2 as a E3-specific ligase) and in vivo. Moreover, we show that ATF7 sumoylation affects its intranuclear localization by delaying its entry into the nucleus. Furthermore, SUMO conjugation inhibits ATF7 transactivation activity by (i) impairing its association with TAF12 and (ii) blocking its binding-to-specific sequences within target promoters.

Links

PubMed PMC1851647 Online version:10.1093/nar/gkl1168

Keywords

Activating Transcription Factors/analysis; Activating Transcription Factors/antagonists & inhibitors; Activating Transcription Factors/metabolism; Cell Line; Cell Nucleus/chemistry; Humans; Molecular Chaperones/metabolism; Nuclear Pore Complex Proteins/metabolism; Protein Processing, Post-Translational; SUMO-1 Protein/metabolism; Ubiquitin-Protein Ligases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:ATF7

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q16514

F

Seeded From UniProt

complete

HUMAN:ATF7

located_in

GO:0005654: nucleoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:ATF7

enables

GO:0000976: transcription cis-regulatory region binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:ATF7

enables

GO:0001223: transcription coactivator binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(UniProtKB:Q16514)

Seeded From UniProt

complete

HUMAN:ATF7

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:RBP2

enables

GO:0061665: SUMO ligase activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(UniProtKB:P17544)
  • has_input:(UniProtKB:P63165)

Seeded From UniProt

complete

HUMAN:SUMO1

involved_in

GO:0016925: protein sumoylation

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(UniProtKB:P17544)

Seeded From UniProt

complete

HUMAN:SUMO1

acts_upstream_of

GO:0010621: negative regulation of transcription by transcription factor localization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SUMO1

involved_in

GO:0042308: negative regulation of protein import into nucleus

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(UniProtKB:P17544)

Seeded From UniProt

complete

HUMAN:TAF12

enables

GO:0003713: transcription coactivator activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(UniProtKB:P17544)

Seeded From UniProt

complete

HUMAN:TAF12

enables

GO:0140297: DNA-binding transcription factor binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:TAF12

enables

GO:0140297: DNA-binding transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P17544

F

Seeded From UniProt

complete

HUMAN:TAF12

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:RBP2

involved_in

GO:0016925: protein sumoylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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