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PMID:17242199

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Citation

Cheng, Y, Buffone, MG, Kouadio, M, Goodheart, M, Page, DC, Gerton, GL, Davidson, I and Wang, PJ (2007) Abnormal sperm in mice lacking the Taf7l gene. Mol. Cell. Biol. 27:2582-9

Abstract

TFIID is a general transcription factor required for transcription of most protein-coding genes by RNA polymerase II. TAF7L is an X-linked germ cell-specific paralogue of TAF7, which is a generally expressed component of TFIID. Here, we report the generation of Taf7l mutant mice by homologous recombination in embryonic stem cells by using the Cre-loxP strategy. While spermatogenesis was completed in Taf7l(-/Y) mice, the weight of Taf7l(-/Y) testis decreased and the amount of sperm in the epididymides was sharply reduced. Mutant epididymal sperm exhibited abnormal morphology, including folded tails. Sperm motility was significantly reduced, and Taf7l(-/Y) males were fertile with reduced litter size. Microarray profiling revealed that the abundance of six gene transcripts (including Fscn1) in Taf7l(-/Y) testes decreased more than twofold. In particular, FSCN1 is an F-action-bundling protein and thus may be critical for normal sperm morphology and sperm motility. Although deficiency of Taf7l may be compensated in part by Taf7, Taf7l has apparently evolved new specialized functions in the gene-selective transcription in male germ cell differentiation. Our mouse studies suggest that mutations in the human TAF7L gene might be implicated in X-linked oligozoospermia in men.

Links

PubMed PMC1899882 Online version:10.1128/MCB.01722-06

Keywords

Animals; Cell Differentiation; Cell Movement; Epididymis/ultrastructure; Female; Fertility/genetics; Gene Expression Profiling; Litter Size; Male; Mice; Mice, Inbred BALB C; Mutation; Oligonucleotide Array Sequence Analysis; Spermatogenesis; Spermatozoa/abnormalities; Spermatozoa/metabolism; Spermatozoa/ultrastructure; Testis/ultrastructure; Transcription Factor TFIID/genetics; Transcription Factor TFIID/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:TBP

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q9R1C0

F

Seeded From UniProt

complete

MOUSE:TBP

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q9D3R9

F

Seeded From UniProt

complete

MOUSE:TBP

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:TAF6

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:TAF5

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:TAF7L

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P29037

F

Seeded From UniProt

complete

MOUSE:TAF7L

acts_upstream_of_or_within

GO:0030317: flagellated sperm motility

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3710399

P

Seeded From UniProt

complete

MOUSE:TAF7L

acts_upstream_of_or_within

GO:0007286: spermatid development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3710399

P

Seeded From UniProt

complete

MOUSE:TAF7

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P29037

F

Seeded From UniProt

complete


See also

References

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