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PMID:17239859

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Citation

Ishii, N, Suga, Y, Hagiya, A, Watanabe, H, Mori, H, Yoshino, M and Tomita, M (2007) Dynamic simulation of an in vitro multi-enzyme system. FEBS Lett. 581:413-20

Abstract

Parameters often are tuned with metabolite concentration time series data to build a dynamic model of metabolism. However, such tuning may reduce the extrapolation ability (generalization capability) of the model. In this study, we determined detailed kinetic parameters of three purified Escherichia coli glycolytic enzymes using the initial velocity method for individual enzymes; i.e., the parameters were determined independently from metabolite concentration time series data. The metabolite concentration time series calculated by the model using the parameters matched the experimental data obtained in an actual multi-enzyme system consisting of the three purified E. coli glycolytic enzymes. Thus, the results indicate that kinetic parameters can be determined without using an undesirable tuning process.

Links

PubMed Online version:10.1016/j.febslet.2006.12.049

Keywords

Escherichia coli/enzymology; Glycolysis; Kinetics; Models, Biological; Multienzyme Complexes/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:G6PI

enables

GO:0004347: glucose-6-phosphate isomerase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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