PMID:17172643

Citation	Shimazu, T, Horinouchi, S and Yoshida, M (2007) Multiple histone deacetylases and the CREB-binding protein regulate pre-mRNA 3'-end processing. J. Biol. Chem. 282:4470-8
Abstract	Trichostatin A (TSA), a specific inhibitor of histone deacetylases (HDACs), induces acetylation of various non-histone proteins such as p53 and alpha-tubulin. We purified several acetylated proteins by the affinity to an anti-acetylated lysine (AcLys) antibody from cells treated with TSA and identified them by mass spectrometry. Here we report on acetylation of CFIm25, a component of mammalian cleavage factor Im (CF Im), and poly(A) polymerase (PAP), a polyadenylating enzyme for the pre-mRNA 3'-end. The residues acetylated in these proteins were mapped onto the regions required for interaction with each other. Whereas CBP acetylated these proteins, HDAC1, HDAC3, HDAC10, SIRT1, and SIRT2 were involved in in vivo deacetylation. Acetylation of the CFIm25 occurred depending on the cleavage factor complex formation. Importantly, the interaction between PAP and CF Im complex was decreased by acetylation. We also demonstrated that acetylation of PAP inhibited the nuclear localization of PAP by inhibiting the binding to the importin alpha/beta complex. These results suggest that CBP and HDACs regulate the 3'-end processing machinery and modulate the localization of PAP through the acetylation and deacetylation cycle.
Links	PubMed Online version:10.1074/jbc.M609745200
Keywords	3' Untranslated Regions/metabolism; Acetylation/drug effects; Animals; COS Cells; CREB-Binding Protein/metabolism; Cercopithecus aethiops; Enzyme Inhibitors/pharmacology; Histone Deacetylase Inhibitors; Histone Deacetylases/metabolism; Humans; Hydroxamic Acids/pharmacology; Polynucleotide Adenylyltransferase/metabolism; Protein Binding/drug effects; Protein Processing, Post-Translational/drug effects; RNA Precursors/metabolism; RNA Processing, Post-Transcriptional/drug effects; RNA Processing, Post-Transcriptional/physiology; Sirtuin 1; Sirtuin 2; Sirtuins/metabolism; Tubulin/metabolism; Tumor Suppressor Protein p53/metabolism; alpha Karyopherins/metabolism; beta Karyopherins; mRNA Cleavage and Polyadenylation Factors/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:HDAC3	enables	GO:0033558: protein lysine deacetylase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:HDAC3	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O43809	F		Seeded From UniProt	complete
HUMAN:CPSF5	enables	GO:0042826: histone deacetylase binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q969S8	F		Seeded From UniProt	complete
HUMAN:CPSF5	enables	GO:0042826: histone deacetylase binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O15379	F		Seeded From UniProt	complete
HUMAN:CPSF5	enables	GO:0042826: histone deacetylase binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q13547	F		Seeded From UniProt	complete
HUMAN:CPSF5	enables	GO:0042826: histone deacetylase binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q8IXJ6	F		Seeded From UniProt	complete
HUMAN:CPSF5	enables	GO:0042826: histone deacetylase binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q96EB6	F		Seeded From UniProt	complete
HUMAN:HDAC1	enables	GO:0033558: protein lysine deacetylase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:HDAC1	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O43809	F		Seeded From UniProt	complete
HUMAN:SIR2	involved_in	GO:0006476: protein deacetylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:SIR2	enables	GO:0033558: protein lysine deacetylase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:SIR2	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O43809	F		Seeded From UniProt	complete
HUMAN:HDA10	involved_in	GO:0006476: protein deacetylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:HDA10	enables	GO:0033558: protein lysine deacetylase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:HDA10	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O43809	F		Seeded From UniProt	complete
HUMAN:SIR1	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O43809	F		Seeded From UniProt	complete
HUMAN:SIRT1	involved_in	GO:0016575: histone deacetylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:HDAC3	enables	GO:0033558: protein deacetylase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:HDAC3	involved_in	GO:0006476: protein deacetylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:SIR1	involved_in	GO:0016575: histone deacetylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:HDAC1	enables	GO:0033558: protein deacetylase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:HDAC1	involved_in	GO:0006476: protein deacetylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.