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PMID:17093926

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Citation

Karlsson, C, Jonsson, M, Asp, J, Brantsing, C, Kageyama, R and Lindahl, A (2007) Notch and HES5 are regulated during human cartilage differentiation. Cell Tissue Res. 327:539-51

Abstract

The molecular mechanisms of cartilage differentiation are poorly understood. In a variety of tissues other than cartilage, members of the basic helix-loop-helix (bHLH) family of transcription factors have been demonstrated to play critical roles in differentiation. We have characterized the human bHLH gene HES5 and investigated its role during chondrogenesis. Blockage of the Notch signaling pathway with a gamma-secretase inhibitor has demonstrated that the human HES5 gene is a downstream marker of Notch signaling in articular chondrocytes. Markers for the Notch signaling pathway significantly decrease during cartilage differentiation in vitro. Cell proliferation assayed by using BrdU has revealed that blockage of Notch signaling is associated with significantly decreased proliferation. Northern blot and reverse transcription/polymerase chain reaction of a panel of various tissues have shown that HES5 is transcribed as a 5.4-kb mRNA that is ubiquitously expressed in diverse fetal and adult tissues. Articular cartilage from HES5(-/-) and wild-type mice has been analyzed by using various histological stains. No differences have been detected between the wild-type and HES5(-/-) mice. Our data thus indicate that the human HES5 gene is coupled to the Notch receptor family, that expression of Notch markers (including HES5) decreases during cartilage differentiation, and that the blockage of Notch signaling is associated with significantly decreased cell proliferation.

Links

PubMed Online version:10.1007/s00441-006-0307-0

Keywords

Adolescent; Adult; Animals; Basic Helix-Loop-Helix Transcription Factors/genetics; Basic Helix-Loop-Helix Transcription Factors/metabolism; Cartilage, Articular/cytology; Cartilage, Articular/metabolism; Cell Differentiation; Cells, Cultured; Chondrocytes/cytology; Chondrocytes/metabolism; Female; Gene Expression Regulation/physiology; Gene Silencing; Humans; Male; Mice; Mice, Knockout; Middle Aged; RNA, Messenger/metabolism; Receptors, Notch/antagonists & inhibitors; Receptors, Notch/genetics; Receptors, Notch/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:HES5

NOT|involved_in

GO:0002062: chondrocyte differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:HES5

involved_in

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:HES5

involved_in

GO:0051216: cartilage development

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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