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PMID:17084357

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Citation

Gallagher, CM and Knoblich, JA (2006) The conserved c2 domain protein lethal (2) giant discs regulates protein trafficking in Drosophila. Dev. Cell 11:641-53

Abstract

Drosophila sensory organ precursor (SOP) cells undergo several rounds of asymmetric cell division to generate the four different cell types that make up external sensory organs. Establishment of different fates among daughter cells of the SOP relies on differential regulation of the Notch pathway. Here, we identify the protein Lethal (2) giant discs (Lgd) as a critical regulator of Notch signaling in the SOP lineage. We show that lgd encodes a conserved C2 domain protein that binds to phospholipids present on early endosomes. When Lgd function is compromised, Notch and other transmembrane proteins accumulate in enlarged early endosomal compartments. These enlarged endosomes are positive for Rab5 and Hrs, a protein involved in trafficking into the degradative pathway. Our experiments suggest that Lgd is a critical regulator of endocytosis that is not present in yeast and acts in the degradative pathway after Hrs.

Links

PubMed Online version:10.1016/j.devcel.2006.09.014

Keywords

Animals; Cell Division; Cell Lineage; Cells, Cultured; Drosophila/growth & development; Drosophila/metabolism; Drosophila/physiology; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila Proteins/physiology; Endosomal Sorting Complexes Required for Transport; Endosomes/metabolism; Larva; Molecular Sequence Data; Mutation; Phospholipids/metabolism; Phosphoproteins/metabolism; Protein Structure, Tertiary; Protein Transport; Receptors, Notch/metabolism; Signal Transduction; Tumor Suppressor Proteins/genetics; Tumor Suppressor Proteins/physiology; rab5 GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:C2D1

enables

GO:0005543: phospholipid binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:C2D1

involved_in

GO:0016197: endosomal transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:C2D1

involved_in

GO:0045035: sensory organ precursor cell division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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