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PMID:17081985

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Citation

Shen, TH, Lin, HK, Scaglioni, PP, Yung, TM and Pandolfi, PP (2006) The mechanisms of PML-nuclear body formation. Mol. Cell 24:331-9

Abstract

PML nuclear bodies (NBs) are nuclear structures that have been implicated in processes such as transcriptional regulation, genome stability, response to viral infection, apoptosis, and tumor suppression. PML has been found to be essential for the formation of the NBs, as these structures do not form in Pml null cells, although PML add back fully rescues their formation. However, the basis for such a structural role of PML is unknown. We demonstrate that PML contains a SUMO binding motif that is independent of its SUMOylation sites and is surprisingly necessary for PML-NB formation. We demonstrate that the PML RING domain is critical for PML SUMOylation and PML-NB formation. We propose a model for PML-NB formation whereby PML SUMOylation and noncovalent binding of PML to SUMOylated PML through the SUMO binding motif constitutes the nucleation event for subsequent recruitment of SUMOylated proteins and/or proteins containing SUMO binding motifs to the PML NBs.

Links

PubMed PMC1978182 Online version:10.1016/j.molcel.2006.09.013

Keywords

Amino Acid Motifs; Amino Acid Sequence; Animals; Cell Line, Transformed; Cell Nucleus Structures/metabolism; Fibroblasts/cytology; Fibroblasts/pathology; Humans; Mice; Models, Biological; Molecular Sequence Data; Nuclear Proteins/chemistry; Nuclear Proteins/deficiency; Nuclear Proteins/metabolism; Protein Binding; Protein Structure, Tertiary; SUMO-1 Protein/metabolism; Transcription Factors/chemistry; Transcription Factors/deficiency; Transcription Factors/metabolism; Tumor Suppressor Proteins/chemistry; Tumor Suppressor Proteins/deficiency; Tumor Suppressor Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PML

located_in

GO:0016605: PML body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:SUMO1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P29590

F

Seeded From UniProt

complete

HUMAN:SUMO1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PML

part_of

GO:0016605: PML body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PML

involved_in

GO:0030578: PML body organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PML

enables

GO:0032183: SUMO binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P63165

F

Seeded From UniProt

complete

See also

References

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