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PMID:17057717

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Citation

Sivanathan, V, Allen, MD, de Bekker, C, Baker, R, Arciszewska, LK, Freund, SM, Bycroft, M, Löwe, J and Sherratt, DJ (2006) The FtsK gamma domain directs oriented DNA translocation by interacting with KOPS. Nat. Struct. Mol. Biol. 13:965-72

Abstract

The bacterial septum-located DNA translocase FtsK coordinates circular chromosome segregation with cell division. Rapid translocation of DNA by FtsK is directed by 8-base-pair DNA motifs (KOPS), so that newly replicated termini are brought together at the developing septum, thereby facilitating completion of chromosome segregation. Translocase functions reside in three domains, alpha, beta and gamma. FtsKalphabeta are necessary and sufficient for ATP hydrolysis-dependent DNA translocation, which is modulated by FtsKgamma through its interaction with KOPS. By solving the FtsKgamma structure by NMR, we show that gamma is a winged-helix domain. NMR chemical shift mapping localizes the DNA-binding site on the gamma domain. Mutated proteins with substitutions in the FtsKgamma DNA-recognition helix are impaired in DNA binding and KOPS recognition, yet remain competent in DNA translocation and XerCD-dif site-specific recombination, which facilitates the late stages of chromosome segregation.

Links

PubMed PMC2556771 Online version:10.1038/nsmb1158

Keywords

Base Sequence; Binding Sites; Chromosomes, Bacterial/metabolism; DNA Helicases/chemistry; DNA Helicases/metabolism; DNA Replication; DNA, Bacterial/chemistry; DNA, Bacterial/metabolism; DNA-Binding Proteins/metabolism; Escherichia coli/chemistry; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Protein Structure, Tertiary; Pseudomonas aeruginosa/chemistry; Pseudomonas aeruginosa/enzymology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:FTSK

enables

GO:0043565: sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

See also

References

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