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PMID:17036048

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Citation

Pelegrin, P and Surprenant, A (2006) Pannexin-1 mediates large pore formation and interleukin-1beta release by the ATP-gated P2X7 receptor. EMBO J. 25:5071-82

Abstract

P2X(7) receptors are ATP-gated cation channels; their activation in macrophage also leads to rapid opening of a membrane pore permeable to dyes such as ethidium, and to release of the pro-inflammatory cytokine, interleukin-1beta (IL-1beta). It has not been known what this dye-uptake path is, or whether it is involved in downstream signalling to IL-1beta release. Here, we identify pannexin-1, a recently described mammalian protein that functions as a hemichannel when ectopically expressed, as this dye-uptake pathway and show that signalling through pannexin-1 is required for processing of caspase-1 and release of mature IL-1beta induced by P2X(7) receptor activation.

Links

PubMed PMC1630421 Online version:10.1038/sj.emboj.7601378

Keywords

Animals; Caspase 1/metabolism; Cell Line; Cell Membrane/metabolism; Connexins/metabolism; Humans; Interleukin-1beta/secretion; Nerve Tissue Proteins/metabolism; Rats; Receptors, Purinergic P2/metabolism; Receptors, Purinergic P2X7; Signal Transduction/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:P2RX7

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q96RD7

F

Seeded From UniProt

complete

HUMAN:PANX1

involved_in

GO:0032731: positive regulation of interleukin-1 beta production

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PANX1

located_in

GO:0032059: bleb

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PANX1

involved_in

GO:0006812: cation transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PANX1

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PANX1

enables

GO:0005102: signaling receptor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q64663

F

Seeded From UniProt

complete

HUMAN:P2RX7

involved_in

GO:0032731: positive regulation of interleukin-1 beta production

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:P2RX7

involved_in

GO:0050718: positive regulation of interleukin-1 beta secretion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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