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PMID:17013749

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Citation

Moeder, W, Del Pozo, O, Navarre, DA, Martin, GB and Klessig, DF (2007) Aconitase plays a role in regulating resistance to oxidative stress and cell death in Arabidopsis and Nicotiana benthamiana. Plant Mol. Biol. 63:273-87

Abstract

In animals, aconitase is a bifunctional protein. When an iron-sulfur cluster is present in its catalytic center, aconitase displays enzymatic activity; when this cluster is lost, it switches to an RNA-binding protein that regulates the translatability or stability of certain transcripts. To investigate the role of aconitase in plants, we assessed its ability to bind mRNA. Recombinant aconitase failed to bind an iron responsive element (IRE) from the human ferritin gene. However, it bound the 5' UTR of the Arabidopsis chloroplastic CuZn superoxide dismutase 2 (CSD2) mRNA, and this binding was specific. Arabidopsis aconitase knockout (KO) plants were found to have significantly less chlorosis after treatment with the superoxide-generating compound, paraquat. This phenotype correlated with delayed induction of the antioxidant gene GST1, suggesting that these KO lines are more tolerant to oxidative stress. Increased levels of CSD2 mRNAs were observed in the KO lines, although the level of CSD2 protein was not affected. Virus-induced gene silencing (VIGS) of aconitase in Nicotiana benthamiana caused a 90% reduction in aconitase activity, stunting, spontaneous necrotic lesions, and increased resistance to paraquat. The silenced plants also had less cell death after transient co-expression of the AvrPto and Pto proteins or the pro-apoptotic protein Bax. Following inoculation with Pseudomonas syringae pv. tabaci carrying avrPto, aconitase-silenced N. benthamiana plants expressing the Pto transgene displayed a delayed hypersensitive response (HR) and supported higher levels of bacterial growth. Disease-associated cell death in N. benthamiana inoculated with P. s. pv. tabaci was also reduced. Taken together, these results suggest that aconitase plays a role in mediating oxidative stress and regulating cell death.

Links

PubMed Online version:10.1007/s11103-006-9087-x

Keywords

Aconitate Hydratase/physiology; Arabidopsis/cytology; Arabidopsis/metabolism; Cell Death/physiology; Oxidative Stress/physiology; Tobacco/cytology; Tobacco/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ACO1

located_in

GO:0005829: cytosol

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

ARATH:ACO1

involved_in

GO:0006102: isocitrate metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ACO1

involved_in

GO:0006101: citrate metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ACO1

enables

GO:0003994: aconitate hydratase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ACO1

enables

GO:0048027: mRNA 5'-UTR binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ACO2M

located_in

GO:0005829: cytosol

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

ARATH:ACO2M

involved_in

GO:0006979: response to oxidative stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ACO2M

involved_in

GO:0006102: isocitrate metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ACO2M

involved_in

GO:0006101: citrate metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ACO2M

enables

GO:0003994: aconitate hydratase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ACO3M

located_in

GO:0005829: cytosol

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

ARATH:ACO3M

involved_in

GO:0006979: response to oxidative stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ACO3M

involved_in

GO:0006102: isocitrate metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ACO3M

involved_in

GO:0006101: citrate metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ACO3M

enables

GO:0003994: aconitate hydratase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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