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PMID:16990135

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Citation

Lee, KH, Piao, HL, Kim, HY, Choi, SM, Jiang, F, Hartung, W, Hwang, I, Kwak, JM, Lee, IJ and Hwang, I (2006) Activation of glucosidase via stress-induced polymerization rapidly increases active pools of abscisic acid. Cell 126:1109-20

Abstract

Abscisic acid (ABA) is a phytohormone critical for plant growth, development, and adaptation to various stress conditions. Plants have to adjust ABA levels constantly to respond to changing physiological and environmental conditions. To date, the mechanisms for fine-tuning ABA levels remain elusive. Here we report that AtBG1, a beta-glucosidase, hydrolyzes glucose-conjugated, biologically inactive ABA to produce active ABA. Loss of AtBG1 causes defective stomatal movement, early germination, abiotic stress-sensitive phenotypes, and lower ABA levels, whereas plants with ectopic AtBG1 accumulate higher ABA levels and display enhanced tolerance to abiotic stress. Dehydration rapidly induces polymerization of AtBG1, resulting in a 4-fold increase in enzymatic activity. Furthermore, diurnal increases in ABA levels are attributable to polymerization-mediated AtBG1 activation. We propose that the activation of inactive ABA pools by polymerized AtBG1 is a mechanism by which plants rapidly adjust ABA levels and respond to changing environmental cues.

Links

PubMed Online version:10.1016/j.cell.2006.07.034

Keywords

Abscisic Acid/biosynthesis; Adaptation, Physiological/physiology; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/physiology; Circadian Rhythm/physiology; Dehydration/enzymology; Environment; Enzyme Activation/physiology; Plants, Genetically Modified/enzymology; Plants, Genetically Modified/genetics; Plants, Genetically Modified/physiology; Polymers/metabolism; Time Factors; Up-Regulation/physiology; beta-Glucosidase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:BGL18

involved_in

GO:0030104: water homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL18

involved_in

GO:0010119: regulation of stomatal movement

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL18

involved_in

GO:0009789: positive regulation of abscisic acid-activated signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL18

involved_in

GO:0009737: response to abscisic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL18

enables

GO:0008422: beta-glucosidase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:BGL18

involved_in

GO:0051258: protein polymerization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL18

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:BGL18

acts_upstream_of_or_within

GO:0009687: abscisic acid metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL18

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL18

acts_upstream_of_or_within

GO:0009414: response to water deprivation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL18

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT1G54200

F

Seeded From UniProt

complete

ARATH:BGL18

enables

GO:0051993: abscisic acid glucose ester beta-glucosidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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