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PMID:16980630

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Citation

Buvelot Frei, S, Rahl, PB, Nussbaum, M, Briggs, BJ, Calero, M, Janeczko, S, Regan, AD, Chen, CZ, Barral, Y, Whittaker, GR and Collins, RN (2006) Bioinformatic and comparative localization of Rab proteins reveals functional insights into the uncharacterized GTPases Ypt10p and Ypt11p. Mol. Cell. Biol. 26:7299-317

Abstract

A striking characteristic of a Rab protein is its steady-state localization to the cytosolic surface of a particular subcellular membrane. In this study, we have undertaken a combined bioinformatic and experimental approach to examine the evolutionary conservation of Rab protein localization. A comprehensive primary sequence classification shows that 10 out of the 11 Rab proteins identified in the yeast (Saccharomyces cerevisiae) genome can be grouped within a major subclass, each comprising multiple Rab orthologs from diverse species. We compared the locations of individual yeast Rab proteins with their localizations following ectopic expression in mammalian cells. Our results suggest that green fluorescent protein-tagged Rab proteins maintain localizations across large evolutionary distances and that the major known player in the Rab localization pathway, mammalian Rab-GDI, is able to function in yeast. These findings enable us to provide insight into novel gene functions and classify the uncharacterized Rab proteins Ypt10p (YBR264C) as being involved in endocytic function and Ypt11p (YNL304W) as being localized to the endoplasmic reticulum, where we demonstrate it is required for organelle inheritance.

Links

PubMed PMC1592887 Online version:10.1128/MCB.02405-05

Keywords

Animals; Cells, Cultured; Computational Biology; Endoplasmic Reticulum/metabolism; Fluorescence; GTP-Binding Proteins/metabolism; Green Fluorescent Proteins/metabolism; Guanine Nucleotide Dissociation Inhibitors/metabolism; HeLa Cells; Humans; Principal Component Analysis; Protein Transport; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/metabolism; rab GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:YPT11

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:YPT11

involved_in

GO:0048309: endoplasmic reticulum inheritance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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