GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16973135

From GONUTS
Jump to: navigation, search
Citation

Besco, JA, Hooft van Huijsduijnen, R, Frostholm, A and Rotter, A (2006) Intracellular substrates of brain-enriched receptor protein tyrosine phosphatase rho (RPTPrho/PTPRT). Brain Res. 1116:50-7

Abstract

Receptor protein tyrosine phosphatase rho (RPTPrho/PTPRT) is a transmembrane protein that is highly expressed in the developing and adult central nervous system. It is a member of the RPTP R2B subfamily, which includes PTPkappa, PTPmu and PCP-2. Glutathione-S-transferase (GST) pulldown assays were used to show that RPTPrho interacts with several adherens junctional proteins in brain, including E-cadherin, N-cadherin, VE-cadherin (cadherin-5), desmoglein, alpha, beta and gamma catenin, p120(ctn) and alpha-actinin. With the exception of E-cadherin and alpha-actinin, binding was considerably reduced at high sodium concentrations. Furthermore, immunoprecipitation phosphatase assays indicated that E-cadherin, and to a far lesser extent p120(ctn), were tyrosine dephosphorylated by a recombinant RPTPrho intracellular fragment, and thus, were likely to be primary substrates for RPTPrho. The interaction of RPTPrho with adherens junctional components suggests that this phosphatase may transduce extracellular signals to the actin cytoskeleton and thereby play a role in regulating cadherin-mediated cell adhesion in the central nervous system.

Links

PubMed Online version:10.1016/j.brainres.2006.07.122

Keywords

Adherens Junctions/metabolism; Brain Chemistry/physiology; Cadherins/metabolism; Catenins/metabolism; Cell Line; DNA, Complementary/biosynthesis; Escherichia coli/metabolism; Glutathione Transferase/metabolism; Humans; Immunoprecipitation; Nerve Tissue Proteins/metabolism; Protein-Tyrosine Kinases/metabolism; Receptor-Like Protein Tyrosine Phosphatases, Class 2; Recombinant Fusion Proteins/biosynthesis

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

See Help:References for how to manage references in GONUTS.