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PMID:16903353

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Citation

Paape, M, Solovyev, AG, Erokhina, TN, Minina, EA, Schepetilnikov, MV, Lesemann, DE, Schiemann, J, Morozov, SY and Kellmann, JW (2006) At-4/1, an interactor of the Tomato spotted wilt virus movement protein, belongs to a new family of plant proteins capable of directed intra- and intercellular trafficking. Mol. Plant Microbe Interact. 19:874-83

Abstract

The Tomato spotted wilt virus (TSWV) encoded NSm movement protein facilitates cell-to-cell spread of the viral genome through structurally modified plasmodesmata. NSm has been utilized as bait in yeast two-hybrid interaction trap screenings. As a result, a protein of unknown function, called At-4/1, was isolated from an Arabidopsis thaliana GAL4 activation domain-tagged cDNA library. Using polyclonal antibodies against bacterially expressed At-4/1, Western blot analysis of protein extracts isolated from different plant species as well as genome database screenings showed that homologues of At-4/1 seemed to be encoded by many vascular plants. For subcellular localization studies, At-4/1 was fused to green fluorescent protein, and corresponding expression vectors were used in particle bombardment and agroinfiltration assays. Confocal laser scannings revealed that At-4/1 assembled in punctate spots at the cell periphery. The protein accumulated intracellularly in a polarized fashion, appearing in only one-half of a bombarded epidermal cell, and, moreover, moved from cell to cell, forming twin-structured bodies seemingly located at both orifices of the plasmodesmatal pore. In coexpression studies, At-4/1 colocalized with a plant virus movement protein TGBp3 known to reside in endoplasmic reticulum-derived membrane structures located in close vicinity to plasmodesmata. Thus, At-4/1 belongs to a new family of plant proteins capable of directed intra- and intercellular trafficking.

Links

PubMed Online version:10.1094/MPMI-19-0874

Keywords

Amino Acid Sequence; Arabidopsis/cytology; Arabidopsis/metabolism; Arabidopsis/virology; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Conserved Sequence; Gene Library; Green Fluorescent Proteins/analysis; Immunohistochemistry; Molecular Sequence Data; Multigene Family; Plant Leaves/metabolism; Plant Leaves/virology; Plant Viral Movement Proteins; Plants, Genetically Modified/cytology; Plants, Genetically Modified/metabolism; Plants, Genetically Modified/virology; Plasmodesmata/metabolism; Protein Transport; Recombinant Fusion Proteins/analysis; Sequence Alignment; Tobacco/cytology; Tobacco/genetics; Tospovirus/metabolism; Two-Hybrid System Techniques; Viral Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

TSWV1:MVP

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q1PE49

F

Seeded From UniProt

complete

TSWV1:MVP

located_in

GO:0044219: host cell plasmodesma

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:4ON1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P36292

F

Seeded From UniProt

complete

ARATH:4ON1

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:4ON1

located_in

GO:0009506: plasmodesma

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:4ON1

involved_in

GO:0010496: intercellular transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:4ON1

involved_in

GO:0046907: intracellular transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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