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PMID:16884685

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Citation

Moon, JC, Jang, HH, Chae, HB, Lee, JR, Lee, SY, Jung, YJ, Shin, MR, Lim, HS, Chung, WS, Yun, DJ, Lee, KO and Lee, SY (2006) The C-type Arabidopsis thioredoxin reductase ANTR-C acts as an electron donor to 2-Cys peroxiredoxins in chloroplasts. Biochem. Biophys. Res. Commun. 348:478-84

Abstract

2-Cys peroxiredoxins (Prxs) play important roles in the antioxidative defense systems of plant chloroplasts. In order to determine the interaction partner for these proteins in Arabidopsis, we used a yeast two-hybrid screening procedure with a C175S-mutant of Arabidopsis 2-Cys Prx-A as bait. A cDNA encoding an NADPH-dependent thioredoxin reductase (NTR) isotype C was identified and designated ANTR-C. We demonstrated that this protein effected efficient transfer of electrons from NADPH to the 2-Cys Prxs of chloroplasts. Interaction between 2-Cys Prx-A and ANTR-C was confirmed by a pull-down experiment. ANTR-C contained N-terminal TR and C-terminal Trx domains. It exhibited both TR and Trx activities and co-localized with 2-Cys Prx-A in chloroplasts. These results suggest that ANTR-C functions as an electron donor for plastidial 2-Cys Prxs and represents the NADPH-dependent TR/Trx system in chloroplasts.

Links

PubMed Online version:10.1016/j.bbrc.2006.07.088

Keywords

Arabidopsis/enzymology; Arabidopsis Proteins/metabolism; Chloroplasts/enzymology; Electrons; NADP/metabolism; Peroxidases/metabolism; Peroxiredoxins; Thioredoxin-Disulfide Reductase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:TRXB3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q96291

F

Seeded From UniProt

complete

ARATH:TRXB3

enables

GO:0008047: enzyme activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:TRXB3

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TRXB3

enables

GO:0016671: oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:TRXB3

enables

GO:0004791: thioredoxin-disulfide reductase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:TRXB3

involved_in

GO:0045454: cell redox homeostasis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TRXB3

involved_in

GO:0043085: positive regulation of catalytic activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BAS1A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O22229

F

Seeded From UniProt

complete


See also

References

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