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PMID:16820281

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Citation

Heo, K, Ha, SH, Chae, YC, Lee, S, Oh, YS, Kim, YH, Kim, SH, Kim, JH, Mizoguchi, A, Itoh, TJ, Kwon, HM, Ryu, SH and Suh, PG (2006) RGS2 promotes formation of neurites by stimulating microtubule polymerization. Cell. Signal. 18:2182-92

Abstract

Regulator of G-protein signaling (RGS) proteins interact with alpha subunits of heterotrimeric G-proteins via the RGS domain and attenuate their activity by accelerating GTPase activity. RGS2, a member of the RGS family, regulates synaptic development via hereto unknown mechanism. In this study, we found that RGS2 directly interacted with tubulin via a short region at the N-terminus: amino acids 41-60. RGS2 enhanced microtubule polymerization in vitro, and the tubulin binding region was necessary and sufficient for this activity. In Vero cells, polymerization of microtubule was stimulated when peptides containing the tubulin binding region were microinjected. Immunocytochemical analysis showed that endogenous RGS2 was localized at the termini of neurites in differentiated PC12 cells. Over-expression of RGS2 enhanced the nerve growth factor-induced neurite outgrowth in PC12 cells, while specific knock-down of endogenous RGS2 suppressed the neurite outgrowth. These findings demonstrate that RGS2 contributes to the neuronal cell differentiation via regulation of microtubule dynamics.

Links

PubMed Online version:10.1016/j.cellsig.2006.05.006

Keywords

Amino Acid Sequence; Animals; Binding Sites/genetics; Brain/metabolism; COS Cells; Cell Differentiation/genetics; Cell Differentiation/physiology; Cercopithecus aethiops; Glutathione Transferase/genetics; Glutathione Transferase/metabolism; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Microscopy, Confocal; Microscopy, Electron; Microtubules/metabolism; Microtubules/ultrastructure; Mutation/genetics; Neurites/metabolism; Neurites/physiology; Neurites/ultrastructure; PC12 Cells; Protein Binding; RGS Proteins/chemistry; RGS Proteins/genetics; RGS Proteins/metabolism; RNA Interference/physiology; RNA, Small Interfering/genetics; Rats; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Transfection/methods; Tubulin/genetics; Tubulin/metabolism; Vero Cells

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:TBB5

enables

GO:0032794: GTPase activating protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:621665

F

Seeded From UniProt

complete

RAT:TBB5

enables

GO:0019904: protein domain specific binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:621665

F

Seeded From UniProt

complete

RAT:RGS2

located_in

GO:0043005: neuron projection

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:RGS2

involved_in

GO:0010976: positive regulation of neuron projection development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:RGS2

part_of

GO:0043005: neuron projection

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:RGS2

enables

GO:0048487: beta-tubulin binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:628596

F

Seeded From UniProt

complete

See also

References

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