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PMID:16806135

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Citation

Sun, G, Bi, N, Li, G, Zhu, X, Zeng, W, Wu, G, Xue, H and Chen, B (2006) Identification of lipid binding and lipoprotein lipase activation domains of human apoAV. Chem. Phys. Lipids 143:22-8

Abstract

ApoAV, a newly discovered apolipoprotein, plays a key role in human triglyceride homeostasis; however, the structure-function correlation of apoAV is not clearly understood. To explore the relationship, wild type and six deletion mutants, that is (AV (Delta(1-51)), AV (Delta(51-128)), AV (Delta(132-188)), AV (Delta(192-238)), AV (Delta(246-299)), AV (Delta(301-343))), of human apoAV expressed in Escherichia coli were studied. All the deleted regions together encompass almost the entire 343 amino acid sequence of wild type apoAV. Circular dichroism spectroscopy showed that the alpha helical content of lipid-free wild type apoAV was 46%. In comparison with wild type apoAV, AV (Delta(192-238)) and AV (Delta(301-343)) displayed significantly decreased lipid binding activities, confirming the importance of these two regions in lipid binding function of apoAV. While, the LPL activation function of apoAV remarkably impaired after deletion of residues 192-238. These findings suggested that the domain (192-238) is absolutely necessary for apoAV in lipid binding and lipoprotein lipase activation.

Links

PubMed Online version:10.1016/j.chemphyslip.2006.04.004

Keywords

Animals; Apolipoproteins/chemistry; Apolipoproteins/genetics; Apolipoproteins/isolation & purification; Apolipoproteins/metabolism; Apolipoproteins A; Cattle; Circular Dichroism; Enzyme Activation; Gene Expression; Humans; Kinetics; Lipid Metabolism; Lipoprotein Lipase/metabolism; Mutation/genetics; Protein Binding; Protein Conformation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:APOA5

enables

GO:0031210: phosphatidylcholine binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:APOA5

involved_in

GO:0050996: positive regulation of lipid catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:APOA5

involved_in

GO:0051006: positive regulation of lipoprotein lipase activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:APOA5

enables

GO:0008047: enzyme activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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