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PMID:16785323

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Citation

Liégeois, S, Benedetto, A, Garnier, JM, Schwab, Y and Labouesse, M (2006) The V0-ATPase mediates apical secretion of exosomes containing Hedgehog-related proteins in Caenorhabditis elegans. J. Cell Biol. 173:949-61

Abstract

Polarized intracellular trafficking in epithelia is critical in development, immunity, and physiology to deliver morphogens, defensins, or ion pumps to the appropriate membrane domain. The mechanisms that control apical trafficking remain poorly defined. Using Caenorhabditis elegans, we characterize a novel apical secretion pathway involving multivesicularbodies and the release of exosomes at the apical plasma membrane. By means of two different genetic approaches, we show that the membrane-bound V0 sector of the vacuolar H+-ATPase (V-ATPase) acts in this pathway, independent of its contribution to the V-ATPase proton pump activity. Specifically, we identified mutations in the V0 "a" subunit VHA-5 that affect either the V0-specific function or the V0+V1 function of the V-ATPase. These mutations allowed us to establish that the V0 sector mediates secretion of Hedgehog-related proteins. Our data raise the possibility that the V0 sector mediates exosome and morphogen release in mammals.

Links

PubMed PMC2063919 Online version:10.1083/jcb.200511072

Keywords

Amino Acid Sequence; Animals; Caenorhabditis elegans/cytology; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/growth & development; Caenorhabditis elegans Proteins/chemistry; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans Proteins/physiology; Hedgehog Proteins; Models, Biological; Molecular Sequence Data; Mutagenesis, Site-Directed; Phenotype; Protein Structure, Tertiary; Protein Transport/physiology; Secretory Vesicles/physiology; Secretory Vesicles/ultrastructure; Sequence Alignment; Trans-Activators/metabolism; Vacuolar Proton-Translocating ATPases/chemistry; Vacuolar Proton-Translocating ATPases/genetics; Vacuolar Proton-Translocating ATPases/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:G5EEK9

located_in

GO:0070382: exocytic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:G5EEK9

located_in

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(WBbt:0007846)|part_of:(WBbt:0005775)

Seeded From UniProt

complete

CAEEL:G5EEK9

located_in

GO:0005771: multivesicular body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:G5EEK9

involved_in

GO:0050891: multicellular organismal water homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:G5EEK9

involved_in

GO:1990182: exosomal secretion

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(WBbt:0007846)

Seeded From UniProt

complete

CAEEL:G5EEK9

involved_in

GO:0040002: collagen and cuticulin-based cuticle development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:Q95X44

located_in

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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