GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16765935

From GONUTS
Jump to: navigation, search
Citation

Catena, R, Ronfani, L, Sassone-Corsi, P and Davidson, I (2006) Changes in intranuclear chromatin architecture induce bipolar nuclear localization of histone variant H1T2 in male haploid spermatids. Dev. Biol. 296:231-238

Abstract

Spermiogenesis entails a major biochemical and morphological restructuring of the germ cell packing the DNA into the condensed spermatid nucleus. H1T2 is a histone H1 variant selectively and transiently expressed in male haploid germ cells during spermiogenesis that specifically localizes to a chromatin domain at the apical pole under the acrosome. We explored the mechanisms determining polar localization of H1T2 in spermatids. In acrosome-deficient round spermatids of hrb -/- and gopc -/- mice, H1T2 localization is not altered, indicating that proper acrosome development is not required for specifying nuclear polarity. In contrast, in late round spermatids from trf2 -/- or hmgb2 -/- mice, a bipolar H1T2 localization was observed revealing that polarity is modified by loss of proteins specifying chromatin architecture. Our results show that intranuclear chromatin organization is critical for correct polar localization of H1T2 and that H1T2 can be a useful molecular marker revealing chromatin disorganization in spermatids.

Links

PubMed Online version:10.1016/j.ydbio.2006.04.458

Keywords

Active Transport, Cell Nucleus/genetics; Animals; Cell Nucleus/genetics; Cell Nucleus/metabolism; Chromatin/metabolism; Chromatin/physiology; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Genetic Markers; Genetic Variation; Haploidy; Male; Mice; Mice, Knockout; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Protein Transport/genetics; Spermatids/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:HMGB2

acts_upstream_of_or_within

GO:0007289: spermatid nucleus differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2387671

P

  • occurs_in:(CL:0000018)

Seeded From UniProt

complete

MOUSE:TBPL1

acts_upstream_of_or_within

GO:0007289: spermatid nucleus differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2181639

P

  • occurs_in:(CL:0000018)

Seeded From UniProt

complete

MOUSE:GOPC

acts_upstream_of_or_within

GO:0007289: spermatid nucleus differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2386794

P

  • occurs_in:(CL:0000018)

Seeded From UniProt

complete

MOUSE:H1FNT

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000018)

Seeded From UniProt

complete

MOUSE:AGFG1

acts_upstream_of_or_within

GO:0007289: spermatid nucleus differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2182772

P

  • occurs_in:(CL:0000018)

Seeded From UniProt

complete

MOUSE:GOPC

acts_upstream_of_or_within

GO:0007289: spermatid nucleus differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2386794

P

occurs_in:(CL:0000018)

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.