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PMID:16751777

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Citation

Aravin, A, Gaidatzis, D, Pfeffer, S, Lagos-Quintana, M, Landgraf, P, Iovino, N, Morris, P, Brownstein, MJ, Kuramochi-Miyagawa, S, Nakano, T, Chien, M, Russo, JJ, Ju, J, Sheridan, R, Sander, C, Zavolan, M and Tuschl, T (2006) A novel class of small RNAs bind to MILI protein in mouse testes. Nature 442:203-7

Abstract

Small RNAs bound to Argonaute proteins recognize partially or fully complementary nucleic acid targets in diverse gene-silencing processes. A subgroup of the Argonaute proteins--known as the 'Piwi family'--is required for germ- and stem-cell development in invertebrates, and two Piwi members--MILI and MIWI--are essential for spermatogenesis in mouse. Here we describe a new class of small RNAs that bind to MILI in mouse male germ cells, where they accumulate at the onset of meiosis. The sequences of the over 1,000 identified unique molecules share a strong preference for a 5' uridine, but otherwise cannot be readily classified into sequence families. Genomic mapping of these small RNAs reveals a limited number of clusters, suggesting that these RNAs are processed from long primary transcripts. The small RNAs are 26-31 nucleotides (nt) in length--clearly distinct from the 21-23 nt of microRNAs (miRNAs) or short interfering RNAs (siRNAs)--and we refer to them as 'Piwi-interacting RNAs' or piRNAs. Orthologous human chromosomal regions also give rise to small RNAs with the characteristics of piRNAs, but the cloned sequences are distinct. The identification of this new class of small RNAs provides an important starting point to determine the molecular function of Piwi proteins in mammalian spermatogenesis.

Links

PubMed Online version:10.1038/nature04916

Keywords

Animals; Animals, Newborn; Argonaute Proteins; Humans; Male; Mice; Mice, Inbred C57BL; Multigene Family/genetics; Proteins/metabolism; RNA/classification; RNA/genetics; RNA/metabolism; Spermatogenesis/genetics; Testis/metabolism; Time Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:PIWL2

involved_in

GO:0034587: piRNA metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:PIWL2

enables

GO:0034584: piRNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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