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PMID:16721662

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Citation

Ahlborn, GJ, Clare, DA, Sheldon, BW and Kelly, RW (2006) Identification of eggshell membrane proteins and purification of ovotransferrin and beta-NAGase from hen egg white. Protein J. 25:71-81

Abstract

Exposure of selected Gram-positive and Gram-negative bacterial pathogens to egg shell membranes (ESM) significantly reduced their thermal resistance and/or inactivated cells. Although the components responsible for this antibacterial activity have not been conclusively identified, several proteins associated with the ESM activity have been identified including beta-N-acetylglucosaminidase, lysozyme and ovotransferrin, with each displaying varying degrees of antibacterial activity. Numerous attempts to purify active fractions of beta-N-acetylglucosaminidase, lysozyme and ovotransferrin from the ESM proved somewhat limited; however, hen egg white (HEW) beta-N-acetylglucosaminidase was purified using a two-step chromatographic procedure, isoelectric focusing followed by cation exchange chromatography. Pure fractions of ovotransferrin were also obtained in the process. SDS-PAGE electrophoresis and Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry were then used to partially characterize the individual protein components. Purified protein fractions such as these will be required in order to fully elucidate the mechanism responsible for the antimicrobial properties associated with the ESM.

Links

PubMed Online version:10.1007/s10930-006-0010-8

Keywords

Acetylglucosaminidase/analysis; Acetylglucosaminidase/isolation & purification; Animals; Anti-Bacterial Agents/analysis; Chickens; Chromatography, Ion Exchange; Conalbumin/analysis; Conalbumin/isolation & purification; Egg Proteins/chemistry; Electrophoresis, Polyacrylamide Gel; Isoelectric Focusing; Membrane Proteins/chemistry; Muramidase/analysis; Ovum/chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:TRFE

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:ENASE

located_in

GO:0005764: lysosome

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:ENASE

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:AVID

enables

GO:0009374: biotin binding

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

CHICK:AVID

involved_in

GO:0019731: antibacterial humoral response

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

CHICK:ENASE

enables

GO:0016787: hydrolase activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:ENASE

part_of

GO:0005764: lysosome

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:ENASE

involved_in

GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:ENASE

part_of

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:ENASE

enables

GO:0016231: beta-N-acetylglucosaminidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:LYSC

involved_in

GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:LYSC

involved_in

GO:0042742: defense response to bacterium

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:LYSC

enables

GO:0016231: beta-N-acetylglucosaminidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:TRFE

part_of

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

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