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PMID:16699516

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Citation

Henderson, IR, Zhang, X, Lu, C, Johnson, L, Meyers, BC, Green, PJ and Jacobsen, SE (2006) Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning. Nat. Genet. 38:721-5

Abstract

Small RNAs have several important biological functions. MicroRNAs (miRNAs) and trans-acting small interfering RNAs (tasiRNAs) regulate mRNA stability and translation, and siRNAs cause post-transcriptional gene silencing of transposons, viruses and transgenes and are important in both the establishment and maintenance of cytosine DNA methylation. Here, we study the role of the four Arabidopsis thaliana DICER-LIKE genes (DCL1-DCL4) in these processes. Sequencing of small RNAs from a dcl2 dcl3 dcl4 triple mutant showed markedly reduced tasiRNA and siRNA production and indicated that DCL1, in addition to its role as the major enzyme for processing miRNAs, has a previously unknown role in the production of small RNAs from endogenous inverted repeats. DCL2, DCL3 and DCL4 showed functional redundancy in siRNA and tasiRNA production and in the establishment and maintenance of DNA methylation. Our studies also suggest that asymmetric DNA methylation can be maintained by pathways that do not require siRNAs.

Links

PubMed Online version:10.1038/ng1804

Keywords

DNA Methylation; Gene Silencing; Mutation; RNA Processing, Post-Transcriptional; RNA, Small Interfering; Ribonuclease III/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4HQG6

involved_in

GO:0010267: production of ta-siRNAs involved in RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DCL2

involved_in

GO:0010216: maintenance of DNA methylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DCL2

involved_in

GO:0010267: production of ta-siRNAs involved in RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DCL3

involved_in

GO:0010216: maintenance of DNA methylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DCL3

involved_in

GO:0010267: production of ta-siRNAs involved in RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DCL4

involved_in

GO:0010216: maintenance of DNA methylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DCL4

involved_in

GO:0010267: production of ta-siRNAs involved in RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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