PMID:16698022

Citation	Tsuda, M, Seong, KH and Aigaki, T (2006) POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila. FEBS Lett. 580:3296-300
Abstract	Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N-terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and Drosophila. Here, we show that POSH forms a complex with Apoptosis-linked gene-2 (ALG-2) and ALG-2-interacting protein (ALIX/AIP1) in a calcium-dependent manner. Overexpression of ALG-2 or ALIX in developing imaginal eye discs resulted in roughened or melanized eyes, respectively. These phenotypes were enhanced by co-overexpression of POSH. We found that overexpression of either gene could induce ectopic JNK activation, suggesting that POSH/ALG-2/ALIX may function together in the regulation of the JNK pathway.
Links	PubMed Online version:10.1016/j.febslet.2006.05.005
Keywords	Animals; Calcium-Binding Proteins/genetics; Calcium-Binding Proteins/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; Drosophila/chemistry; Drosophila/growth & development; Drosophila/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Eye/anatomy & histology; Eye/growth & development; Eye/metabolism; Gene Expression Regulation, Developmental; Immunoprecipitation; MAP Kinase Kinase 4/metabolism; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Protein Interaction Mapping; RNA, Messenger/analysis; RNA, Messenger/metabolism
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