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PMID:16684770

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Citation

Dietz, ML, Bernaciak, TM, Vendetti, F, Kielec, JM and Hildebrand, JD (2006) Differential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins. J. Biol. Chem. 281:20542-54

Abstract

Shroom is an actin-associated determinant of cell morphology that is required for neural tube closure in both mice and frogs. Shroom regulates this process by causing apical constriction of epithelial cells via a pathway involving myosin II. Here we report on characterization of the Shroom-related proteins Apxl and KIAA1202 and their role in cell architecture. Shroom, Apxl, and KIAA1202 exhibit differing abilities to interact with the actin cytoskeleton. In fibroblasts, Shroom readily associates with actin stress fibers and induces bundling, Apxl is found on cortical actin, and KIAA1202 is localized to a cytoplasmic population of F-actin. In epithelial cells, Apxl and KIAA1202 do not induce apical constriction as Shroom does, but have the capacity to do so if targeted to the apical junctional complex. To determine whether the activity of Shroom-like proteins is conserved in invertebrates, we have tested the ability of the lone Shroomrelated protein in Drosophila, CG8603, to activate the constriction pathway. A chimeric protein consisting of the Shroom targeting domain and the Drosophila protein elicits constriction. Finally, we show that Apxl is involved in regulating the cytoskeletal organization and architecture of endothelial cells. We predict that the ability of Shroom-like proteins to regulate cellular morphology is conserved in evolution and is regulated in part by subcellular localization.

Links

PubMed Online version:10.1074/jbc.M512463200

Keywords

Actins/metabolism; Amino Acid Sequence; Animals; Binding Sites; Cell Line; Cloning, Molecular; Conserved Sequence; Dogs; Endothelium, Vascular/physiology; Evolution, Molecular; Gene Expression Regulation; Invertebrates; Mice; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Molecular Sequence Data; Muscle, Skeletal/physiology; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Vertebrates

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:SHRM

located_in

GO:0030864: cortical actin cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:SHRM

involved_in

GO:0000902: cell morphogenesis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SHRM2

enables

GO:0003779: actin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:SHRM2

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SHRM2

enables

GO:0008013: beta-catenin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:SHRM2

NOT|involved_in

GO:0051017: actin filament bundle assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SHRM2

located_in

GO:0005912: adherens junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SHRM2

located_in

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SHRM2

located_in

GO:0005856: cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SHRM2

colocalizes_with

GO:0030864: cortical actin cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SHRM2

located_in

GO:0005923: bicellular tight junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SHRM2

NOT|involved_in

GO:0000902: cell morphogenesis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SHRM2

enables

GO:0051015: actin filament binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:SHRM2

involved_in

GO:0016477: cell migration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SHRM2

colocalizes_with

GO:0030864: cortical actin cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:SHRM2

NOT|involved_in

GO:0000902: cell morphogenesis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SHRM2

enables

GO:0008013: beta-catenin binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:A2ALU4

F

Seeded From UniProt

complete

HUMAN:SHRM2

NOT|involved_in

GO:0051017: actin filament bundle assembly

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:A2ALU4

P

Seeded From UniProt

complete

HUMAN:SHRM2

located_in

GO:0005912: adherens junction

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:A2ALU4

C

Seeded From UniProt

complete

HUMAN:SHRM2

located_in

GO:0016324: apical plasma membrane

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:A2ALU4

C

Seeded From UniProt

complete

HUMAN:SHRM2

colocalizes_with

GO:0030864: cortical actin cytoskeleton

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:A2ALU4

C

Seeded From UniProt

complete

HUMAN:SHRM2

NOT|involved_in

GO:0000902: cell morphogenesis

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:A2ALU4

P

Seeded From UniProt

complete

HUMAN:SHRM2

enables

GO:0051015: actin filament binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:A2ALU4

F

Seeded From UniProt

complete

MOUSE:SHRM4

colocalizes_with

GO:0030864: cortical actin cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SHRM4

NOT|involved_in

GO:0000902: cell morphogenesis

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q9ULL8

P

Seeded From UniProt

complete

HUMAN:SHRM1

involved_in

GO:0051017: actin filament bundle assembly

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q5SX79

P

Seeded From UniProt

complete

HUMAN:SHRM1

enables

GO:0045159: myosin II binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q5SX79

F

Seeded From UniProt

complete

HUMAN:SHRM1

involved_in

GO:0000902: cell morphogenesis

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q5SX79

P

Seeded From UniProt

complete

HUMAN:SHRM1

enables

GO:0051015: actin filament binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q5SX79

F

Seeded From UniProt

complete

MOUSE:SHRM1

involved_in

GO:0051017: actin filament bundle assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SHRM1

involved_in

GO:0000902: cell morphogenesis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SHRM1

enables

GO:0045159: myosin II binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:SHRM1

enables

GO:0051015: actin filament binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:SHRM4

involved_in

GO:0030036: actin cytoskeleton organization

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SHRM4

located_in

GO:0005884: actin filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:SHRM4

NOT|involved_in

GO:0000902: cell morphogenesis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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