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PMID:16680092
| Citation |
Amar, N, Lustig, G, Ichimura, Y, Ohsumi, Y and Elazar, Z (2006) Two newly identified sites in the ubiquitin-like protein Atg8 are essential for autophagy. EMBO Rep. 7:635-42 |
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| Abstract |
Atg8, a member of a novel ubiquitin-like protein family, is an essential component of the autophagic machinery in yeast. This protein undergoes reversible conjugation to phosphatidylethanolamine through a multistep process in which cleavage of Atg8 by a specific protease is followed by ubiquitin-like conjugation processes. Here, we identify two essential sites in Atg8, one of them involving residues Phe 77 and Phe 79 and the other, located on the opposite surface of Atg8, residues Tyr 49 and Leu 50. We show that these two sites are associated with different functions of Atg8: Phe 77 and Phe 79 seem to be part of the recognition site for Atg4, a cystein protease that acts also as a deubiquitination enzyme, whereas Tyr 49 and Leu 50 act downstream of the lipidation step. These two newly identified distinct sites that are essential for Atg8 activity provide an explanation for the many protein-protein interactions of this low-molecular-weight protein. |
| Links |
PubMed PMC1479593 Online version:10.1038/sj.embor.7400698 |
| Keywords |
Amino Acid Sequence; Amino Acid Substitution; Autophagy; Binding Sites; Green Fluorescent Proteins/metabolism; Microtubule-Associated Proteins/chemistry; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Models, Molecular; Molecular Sequence Data; Phenylalanine/metabolism; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Secondary; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Sequence Homology, Amino Acid; Ubiquitin/chemistry; Vacuoles/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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