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PMID:16672598

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Citation

Pflock, M, Finsterer, N, Joseph, B, Mollenkopf, H, Meyer, TF and Beier, D (2006) Characterization of the ArsRS regulon of Helicobacter pylori, involved in acid adaptation. J. Bacteriol. 188:3449-62

Abstract

The human gastric pathogen Helicobacter pylori is extremely well adapted to the highly acidic conditions encountered in the stomach. The pronounced acid resistance of H. pylori relies mainly on the ammonia-producing enzyme urease; however, urease-independent mechanisms are likely to contribute to acid adaptation. Acid-responsive gene regulation is mediated at least in part by the ArsRS two-component system consisting of the essential OmpR-like response regulator ArsR and the nonessential cognate histidine kinase ArsS, whose autophosphorylation is triggered in response to low pH. In this study, by global transcriptional profiling of an ArsS-deficient H. pylori mutant grown at pH 5.0, we define the ArsR approximately P-dependent regulon consisting of 109 genes, including the urease gene cluster, the genes encoding the aliphatic amidases AmiE and AmiF, and the rocF gene encoding arginase. We show that ArsR approximately P controls the acid-induced transcription of amiE and amiF by binding to extended regions located upstream of the -10 box of the respective promoters. In contrast, transcription of rocF is repressed by ArsR approximately P at neutral, acidic, and mildly alkaline pH via high-affinity binding of the response regulator to a site overlapping the promoter of the rocF gene.

Links

PubMed PMC1482845 Online version:10.1128/JB.188.10.3449-3462.2006

Keywords

Adaptation, Physiological; Bacterial Proteins/genetics; Base Sequence; DNA Primers; Gene Expression Regulation, Bacterial; Helicobacter pylori/genetics; Helicobacter pylori/growth & development; Hydrogen-Ion Concentration; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Open Reading Frames; RNA, Bacterial/genetics; RNA, Bacterial/isolation & purification; Regulon; Restriction Mapping; Trans-Activators/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HELPY:ARSR

part_of

GO:0032993: protein-DNA complex

ECO:0005631: DNAse footprinting evidence used in manual assertion

RefSeq:NC_000915.1

C

Seeded From UniProt

complete

HELPY:ARSR

enables

GO:0000976: transcription regulatory region sequence-specific DNA binding

ECO:0005631: DNAse footprinting evidence used in manual assertion

RefSeq:NC_000915.1

F

Seeded From UniProt

complete

HELPY:ARSR

enables

GO:0001216: DNA-binding transcription activator activity

ECO:0005631: DNAse footprinting evidence used in manual assertion

RefSeq:NC_000915.1

F

Seeded From UniProt

complete

HELPY:ARSR

enables

GO:0001217: DNA-binding transcription repressor activity

ECO:0005631: DNAse footprinting evidence used in manual assertion

RefSeq:NC_000915.1

F

Seeded From UniProt

complete

HELPY:ARSR

involved_in

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0005653: northern assay evidence used in manual assertion

P

Seeded From UniProt

complete

HELPY:ARSR

involved_in

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0005657: primer extension assay evidence used in manual assertion

P

Seeded From UniProt

complete

HELPY:ARSR

involved_in

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0005657: primer extension assay evidence used in manual assertion

P

Seeded From UniProt

complete

HELPJ:Q9ZMR6

GO:0006351: transcription, DNA-dependent

ECO:0000315:

P

Figure 2: pH-responsive transcription of the amidase genes amiE and amiF are mediated mainly by the ArsRS two-component system. ArsS-deficient mutant strains, in which the response regulator ArsR cannot be phosphorylated to affect gene expression (no ArsR~P), showed significantly decreased transcription of amiE and amiF in neutral pH conditions and acidic pH (5.0) conditions compared to wild-type strains.

complete
CACAO 5609


See also

References

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