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PMID:16649143

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Citation

Thacker, C, Sheps, JA and Rose, AM (2006) Caenorhabditis elegans dpy-5 is a cuticle procollagen processed by a proprotein convertase. Cell. Mol. Life Sci. 63:1193-204

Abstract

Genetic analysis of the nematode Caenorhabditis elegans reveals that all dpy-5 alleles are dominant suppressors of bli-4 blistering. Molecular cloning of dpy-5 establishes that it encodes a cuticle procollagen, defects in which are responsible for the short-body, dumpy phenotype. The null mutation, e907 removes the entire coding region, whereas the dpy-5 reference allele, e61, contains a nonsense substitution. RT-PCR analysis and a dpy-5::gfp fusion show that dpy-5 is expressed only in hypodermal cells at all post-embryonic life-cycle stages. Variable expression of dpy-5 in V lineage-derived seam cells suggests an alternative regulatory mechanism in these cells. The dpy-5 gene product contains an Arg-X-X-Arg cleavage motif that could be recognized by a proprotein convertase, such as BLI-4. Mutation of this site cause a dominant dumpy phenotype suggesting Dpy-5 procollagen requires processing for normal cuticle production.

Links

PubMed Online version:10.1007/s00018-006-6012-z

Keywords

Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Molecular Sequence Data; Mutation; Procollagen/genetics; Procollagen/metabolism; Proprotein Convertases/genetics; Proprotein Convertases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:DPY5

located_in

GO:0060102: collagen and cuticulin-based cuticle extracellular matrix

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:DPY5

involved_in

GO:0040014: regulation of multicellular organism growth

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00142941

P

Seeded From UniProt

complete

CAEEL:DPY5

involved_in

GO:0040002: collagen and cuticulin-based cuticle development

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00142941

P

Seeded From UniProt

complete


See also

References

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