GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16648250

From GONUTS
Jump to: navigation, search
Citation

Takayasu, S, Sakurai, T, Iwasaki, S, Teranishi, H, Yamanaka, A, Williams, SC, Iguchi, H, Kawasawa, YI, Ikeda, Y, Sakakibara, I, Ohno, K, Ioka, RX, Murakami, S, Dohmae, N, Xie, J, Suda, T, Motoike, T, Ohuchi, T, Yanagisawa, M and Sakai, J (2006) A neuropeptide ligand of the G protein-coupled receptor GPR103 regulates feeding, behavioral arousal, and blood pressure in mice. Proc. Natl. Acad. Sci. U.S.A. 103:7438-43

Abstract

Here, we report the isolation and characterization of an endogenous peptide ligand of GPR103 from rat brains. The purified peptide was found to be the 43-residue RF-amide peptide QRFP. We also describe two mouse homologues of human GPR103, termed mouse GPR103A and GPR103B. QRFP binds and activates the human GPR103, as well as mouse GPR103A and GPR103B, with nanomolar affinities in transfected cells. Systematic in situ hybridization analysis in mouse brains showed that QRFP is expressed exclusively in the periventricular and lateral hypothalamus, whereas the two receptor mRNAs are distinctly localized in various brain areas without an overlap to each other. When administered centrally in mice, QRFP induced feeding behavior, accompanied by increased general locomotor activity and metabolic rate. QRFP-induced food intake was abolished by preadministration of BIBP3226, a specific antagonist for the Y1 neuropeptide Y receptor. Hypothalamic prepro-QRFP mRNA expression was up-regulated upon fasting and in genetically obese ob/ob and db/db mice. Central QRFP administration also evoked highly sustained elevation of blood pressure and heart rate. Our findings suggest that QRFP and GPR103A/B may regulate diverse neuroendocrine and behavioral functions and implicate this neuropeptide system in metabolic syndrome.

Links

PubMed PMC1464357 Online version:10.1073/pnas.0602371103

Keywords

Amino Acid Sequence; Animal Feed; Animals; Arousal/physiology; Behavior, Animal/physiology; Blood Pressure/drug effects; Brain/metabolism; Humans; Ligands; Locomotion/drug effects; Mice; Neuropeptides/administration & dosage; Neuropeptides/chemistry; Neuropeptides/genetics; Neuropeptides/metabolism; RNA, Messenger/genetics; Rats; Receptors, G-Protein-Coupled/metabolism; Up-Regulation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:OX26

enables

GO:0031854: orexigenic neuropeptide QRFP receptor binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:OX26

involved_in

GO:0007626: locomotory behavior

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:OX26

involved_in

GO:0007625: grooming behavior

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:OX26

involved_in

GO:0060259: regulation of feeding behavior

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:OX26

involved_in

GO:0045777: positive regulation of blood pressure

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.